HBsAg adr CHO
Description
Structure and Functional Characteristics
HBsAg adr CHO is derived from the S-gene of Hepatitis B Virus (HBV), encoding the small envelope protein. Key structural features include:
The protein adopts a transmembrane topology with luminal and cytosolic loops. Its external loop harbors major epitopes critical for immune recognition .
Production and Purification
HBsAg adr CHO is expressed in CHO cells and purified via proprietary chromatographic methods.
CHO systems enable proper glycosylation and disulfide bond formation, mimicking native HBsAg structures .
Applications in Research and Diagnostics
HBsAg adr CHO is utilized in diverse experimental and clinical settings:
Diagnostic Assays
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ELISA: Serves as a capture/conjugate antigen for detecting anti-HBs antibodies .
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Immunochromatography: Used in rapid diagnostic kits for HBsAg detection .
Vaccine Development
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Antigen Source: Combined with pre-S1/S2-containing HBsAg proteins in vaccines to enhance immunogenicity .
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Adjuvant Studies: Demonstrated 10× higher antibody induction when paired with novel adjuvants (e.g., L-pampo) compared to alum .
Antibody Production
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Immunogen: Used to generate monoclonal antibodies (mAbs) targeting HBsAg epitopes .
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B Cell Cloning: Served as a selection antigen for identifying broadly neutralizing antibodies (bnAbs) in HBV-infected/vaccinated individuals .
Vaccine Efficacy
| Study | Key Results | Source |
|---|---|---|
| CHO-Produced HBsAg | Induced robust anti-HBs responses in HBV transgenic mice | |
| Pre-S-Containing Particles | Filamentous structures enhanced immunogenicity in murine models |
Neutralizing Antibodies
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Broad Reactivity: 20 bnAbs isolated from HBV-infected individuals showed EC₅₀ values of 18–350 ng/mL against HBsAg adr CHO, confirming broad neutralization potential .
Comparative Analysis with Other Subtypes
Product Specs
Product Science Overview
Hepatitis B Surface Antigen (HBsAg) is a protein found on the surface of the Hepatitis B virus (HBV). It plays a crucial role in the virus’s ability to infect liver cells and is a key target for the immune response. The “adr” subtype refers to a specific antigenic determinant on the surface antigen, which is used to classify different strains of HBV. The recombinant form of HBsAg, produced in Chinese Hamster Ovary (CHO) cells, is used in various research and diagnostic applications.
HBV is a small, enveloped DNA virus belonging to the Hepadnaviridae family. It has a partially double-stranded circular DNA genome that encodes several proteins, including the surface antigen (HBsAg), core antigen (HBcAg), and e antigen (HBeAg). HBV infection can lead to acute and chronic liver diseases, including cirrhosis and hepatocellular carcinoma.
HBsAg is the primary protein on the surface of HBV and is essential for the virus’s ability to infect liver cells. It is also the main target for the immune response against HBV. There are several subtypes of HBsAg, including adw, adr, ayw, and ayr, which are distinguished by specific antigenic determinants. The “adr” subtype is one of the most common and is used in various research and diagnostic applications.
Recombinant HBsAg is produced using genetic engineering techniques. The gene encoding HBsAg is inserted into CHO cells, which are then cultured to produce the protein. The recombinant protein is purified using chromatographic techniques to ensure high purity and quality. The molecular weight of recombinant HBsAg is approximately 23-27 kDa .
Recombinant HBsAg is used in various research and diagnostic applications, including:
