Product List

Avidin Protein

Avidin

Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin. The Avidin is purified by affinity chromatographic techniques.
The purification procedure ensures minimal contamination by other proteins or DNA.
The resulting high activity and purity of the product gives very low non-specific binding (NSB).
Shipped with Ice Packs
Cat. No.
BT29175
Source
Hen's egg white.
Appearance
Sterile Filtered white lyophilized powder.

Avidin Recombinant

Avidin Recombinant

Recombinant Avidin produced in Plants is a polypeptide chain having a molecular mass of 66kDa and 16kda per subunit.

The Recombinant Avidin is purified by affinity chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT29262
Source

Corn (Zea Mays).

Appearance

Sterile Filtered white lyophilized powder.

Introduction

Definition and Classification

Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles, and amphibians, and deposited in the whites of their eggs . It belongs to the avidin family, which also includes streptavidin, a related protein found in certain bacteria . Avidin is known for its high affinity and specificity for biotin (Vitamin B7), forming one of the strongest known non-covalent bonds .

Biological Properties

Key Biological Properties: Avidin is a glycoprotein with a molecular weight of approximately 66-69 kDa in its tetrameric form . Each subunit can bind one biotin molecule, making it capable of binding up to four biotin molecules simultaneously . The protein is highly stable and functional under various pH and temperature conditions .

Expression Patterns and Tissue Distribution: Avidin is primarily expressed in the oviducts of birds, reptiles, and amphibians, and is deposited in the egg whites . In chickens, avidin makes up approximately 0.05% of the total protein content in egg whites . It is also found in some bacterial species, where it serves similar biotin-binding functions .

Biological Functions

Primary Biological Functions: The primary function of avidin is to bind biotin with high affinity and specificity . This binding capability is utilized in various biochemical and biophysical applications, including protein purification and detection .

Role in Immune Responses and Pathogen Recognition: Avidin is believed to play a role in protecting eggs from bacterial contamination by sequestering biotin, which is essential for bacterial growth . This antimicrobial property helps to inhibit the growth of competing bacteria, acting similarly to an antibiotic .

Modes of Action

Mechanisms with Other Molecules and Cells: Avidin binds biotin through its homotetrameric structure, with each subunit capable of binding one biotin molecule . This interaction is highly specific and forms a stable complex that is resistant to various denaturing conditions .

Binding Partners and Downstream Signaling Cascades: Avidin primarily interacts with biotinylated molecules, including enzymes, antibodies, peptides, and nucleic acids . These interactions are exploited in various laboratory techniques, such as immunohistochemistry (IHC), ELISA, and Western blotting .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of avidin is regulated at the transcriptional level, with specific genes encoding for the protein . Post-translational modifications, such as glycosylation, also play a role in the stability and functionality of avidin .

Transcriptional Regulation and Post-Translational Modifications: Avidin undergoes glycosylation, which contributes to approximately 10% of its molecular weight . This modification involves the addition of carbohydrate moieties, including mannose and N-acetylglucosamine residues .

Applications

Biomedical Research: Avidin’s high affinity for biotin is widely utilized in various research applications, including protein purification, detection, and assay technologies . It is commonly used in affinity purification, immunoprecipitation, and cell surface labeling .

Diagnostic Tools: Avidin-biotin interactions are employed in diagnostic assays such as ELISA, Western blotting, and immunohistochemistry . These techniques leverage the strong binding affinity of avidin for biotinylated targets to achieve high sensitivity and specificity.

Therapeutic Strategies: Avidin is also explored in therapeutic strategies, including drug delivery systems and targeted therapies . Its ability to form stable complexes with biotinylated molecules makes it a valuable tool in nanomedicine and targeted drug delivery.

Role in the Life Cycle

Role Throughout the Life Cycle: Avidin plays a crucial role in the early stages of development by protecting eggs from bacterial contamination . Its antimicrobial properties help ensure the viability of the embryo during development. Additionally, avidin’s role in biotin sequestration may have implications for immune function and pathogen recognition throughout the life cycle .

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