GDNF Rat

Glial-Derived Neurotrophic Factor Rat Recombinant

Recombinant Rat GDNF is a non-glycosylated homodimeric protein. Manufactured using a recombinant E. coli expression system, it contains 2 x 134 amino acid chains and has a molecular weight of 29.8 kDa.
Shipped with Ice Packs
Cat. No.
BT8423
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

GFRA1 Human

GDNF Family Receptor Alpha 1 Human Recombinant

Recombinant human GFRA1, produced in HEK293 cells, is a single polypeptide chain that has undergone glycosylation. It spans amino acid residues 25 to 423 (a.a 25-423), resulting in a protein with 409 amino acids, including a 10 amino acid C-terminal His tag. The calculated molecular mass of this protein is 46.0 kDa.

Shipped with Ice Packs
Cat. No.
BT8488
Source

HEK293 cells.

Appearance
White powder, lyophilized (freeze-dried), and filtered.

GFRA1 Rat

GDNF Family Receptor Alpha 1 Rat Recombinant

Recombinant GFRA1 Rat, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 645 amino acids (25-430a.a.). It has a molecular mass of 72.3kDa (Molecular size on SDS-PAGE appears between 70-100kDa approximately). GFRA1 is expressed with a 239 amino acid hIgG-His tag at the C-Terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT8551
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered colorless solution.

GFRA3 Human

GDNF Family Receptor Alpha 3 Human Recombinant

Recombinant human GFRA3 protein was produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 366 amino acids (residues 32-374) and has a molecular weight of 40.7 kDa. The GFRA3 protein is fused to a 23 amino acid His-tag at the N-terminus and is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT8662
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.

GFRA3 Human, Sf9

GDNF Family Receptor Alpha 3 Human Recombinant, Sf9

Recombinant Human GFRA3, produced in Sf9 Baculovirus cells, is a single glycosylated polypeptide chain. It comprises 585 amino acids (32-374) with a molecular mass of 65.5 kDa. Note: On SDS-PAGE, the molecular size appears approximately between 70-100 kDa. GFRA3 is fused to a 239 amino acid hIgG-His-Tag at its C-terminus and purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT8754
Source
Sf9, Baculovirus cells.
Appearance
A clear, sterile-filtered solution.

GDNF Human

Glial-Derived Neurotrophic Factor Human Recombinant

Recombinant Human Glial Derived Neurotrophic Factor, a protein produced in E. coli, is a non-glycosylated homodimer with two identical chains connected by a disulfide bond. Each chain contains 135 amino acids, totaling a molecular weight of approximately 30kDa. The protein is purified using advanced chromatographic methods to ensure its high quality and purity.
Shipped with Ice Packs
Cat. No.
BT8220
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

GDNF Human, Sf9

Glial-Derived Neurotrophic Factor Human Recombinant, Sf9

Recombinant Human GDNF, produced in Baculovirus, is a single glycosylated polypeptide chain consisting of 113 amino acids (109-211 aa). It has a molecular weight of 12.8 kDa. The protein includes a 10 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT8307
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution that has been sterilized by filtration.

GDNF Mouse

Glial-Derived Neurotrophic Factor Mouse Recombinant

Recombinant Mouse GDNF, produced in E. coli, is a non-glycosylated homodimer protein. It consists of two identical polypeptide chains, each containing 135 amino acids, resulting in a total molecular mass of 30.2 kDa. The protein is purified using proprietary chromatographic techniques to ensure high purity and homogeneity.
Shipped with Ice Packs
Cat. No.
BT8369
Source
Escherichia Coli.
Appearance
The product is supplied as a sterile, white powder that has been lyophilized (freeze-dried).
Definition and Classification

Glial cell line-derived neurotrophic factor (GDNF) is a protein encoded by the GDNF gene in humans. It belongs to the GDNF family of ligands (GFL), which also includes neurturin (NRTN), artemin (ARTN), and persephin (PSPN) . GDNF is classified as a neurotrophic factor, which means it promotes the survival, development, and function of neurons .

Biological Properties

Key Biological Properties: GDNF is a small protein that potently promotes the survival of various types of neurons, particularly dopaminergic neurons . It signals through GFRα receptors, especially GFRα1 .

Expression Patterns and Tissue Distribution: GDNF is highly distributed throughout both the peripheral and central nervous systems. It can be secreted by astrocytes, oligodendrocytes, Schwann cells, motor neurons, and skeletal muscle during the development and growth of neurons and other peripheral cells .

Biological Functions

Primary Biological Functions: GDNF is crucial for the survival of dopaminergic and motor neurons. It prevents apoptosis in motor neurons during development, decreases the overall loss of neurons, rescues cells from axotomy-induced death, and prevents chronic degeneration .

Role in Immune Responses and Pathogen Recognition: While GDNF’s primary role is in neuronal survival and development, it also has functions outside the nervous system, such as in kidney morphogenesis and spermatogenesis .

Modes of Action

Mechanisms with Other Molecules and Cells: GDNF binds to GFRα1, forming a complex that interacts with the RET receptor tyrosine kinase . This interaction activates several intracellular signaling pathways, including the ERK-1 and ERK-2 isoforms of MAP kinase, P13K/AKT pathways, and Src-family kinases .

Binding Partners and Downstream Signaling Cascades: GDNF can also signal through the neural cell adhesion molecule (NCAM) in cells lacking RET, activating Fyn and FAK .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The activity of GDNF is mediated by the RET receptor tyrosine kinase, and GDNF must be bound to GFRα1 for the receptor to modulate its activity . The neurotrophic effect of GDNF requires the presence of transforming growth factor β, which activates the transport of GFRα1 to the cell membrane .

Transcriptional Regulation and Post-Translational Modifications: GDNF is first synthesized as a precursor protein (pro-GDNF) and undergoes a series of protein cleavage and processing to become the active and mature form .

Applications

Biomedical Research: GDNF has been extensively studied for its potential therapeutic applications in neurodegenerative diseases, particularly Parkinson’s disease . It has shown promise in protecting and regenerating dopamine-producing brain cells .

Diagnostic Tools and Therapeutic Strategies: GDNF is being explored as a therapeutic agent delivered through gene therapy or direct protein infusion to treat Parkinson’s disease .

Role in the Life Cycle

Role Throughout the Life Cycle: GDNF plays a critical role in the development, survival, and maintenance of neurons from embryonic stages through adulthood . It supports the survival of dopaminergic and motor neurons, prevents apoptosis, and promotes neuronal health and survival .

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