Procalcitonin Human, His

Procalcitonin Human Recombinant, His Tag

Recombinant Human Procalcitonin, synthesized in E. coli, is a single, non-glycosylated polypeptide chain comprising 114 amino acids (fragment 3-116). With a molecular weight of 17.13 kDa, it features an N-terminal hexahistidine tag and undergoes purification using standard chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT17934
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.

Procalcitonin Mouse

Procalcitonin Mouse Recombinant

Recombinant Mouse Procalcitonin, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Val26 to Ser136 (totaling 121 amino acids), including a 10-amino acid His tag located at the N-terminus. The calculated molecular mass is 13.6 kDa.
Shipped with Ice Packs
Cat. No.
BT18041
Source
Escherichia Coli.
Appearance
White lyophilized (freeze-dried) powder after filtration.

Procalcitonin Porcine

Procalcitonin Porcine Recombinant

Recombinant Porcine Procalcitonin, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Thr26 to Asn141 (126 amino acids total), including a 10 amino acid His tag at the N-terminus. The calculated molecular mass is 14.1 kDa.
Shipped with Ice Packs
Cat. No.
BT18126
Source
Escherichia Coli.
Appearance
White, lyophilized (freeze-dried) powder after filtration.

Procalcitonin Rat

Procalcitonin Rat Recombinant

Recombinant Rat Procalcitonin, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Val26 to Asn136 (totaling 121 amino acids). It includes a 10 amino acid His tag located at the N-terminus. The calculated molecular mass of this protein is 13.6 kDa.
Shipped with Ice Packs
Cat. No.
BT18217
Source
Escherichia Coli.
Appearance
White lyophilized powder that has been filtered.

Procalcitonin Rhesus

Procalcitonin Rhesus Recombinant

Recombinant Rhesus Procalcitonin, produced in E. coli, is a single, non-glycosylated polypeptide chain (Ala26-Asn140) consisting of 125 amino acids. This includes a 10 amino acid His tag located at the N-terminus. The calculated molecular mass is 14 kDa.
Shipped with Ice Packs
Cat. No.
BT18284
Source
Escherichia Coli.
Appearance
White lyophilized (freeze-dried) powder, filtered for appearance.

Procalcitonin Canine

Procalcitonin Canine Recombinant

Recombinant Canine Procalcitonin, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Ala26 to Arg130 (totaling 115 amino acids), including a 10 amino acid His tag at the N-terminus. The calculated molecular mass is 12.7 kDa.
Shipped with Ice Packs
Cat. No.
BT17737
Source
Escherichia Coli.
Appearance
Lyophilized powder with a white, filtered appearance.

Procalcitonin Human

Procalcitonin Human Recombinant

Produced in E. coli, Recombinant Human Procalcitonin is a single, non-glycosylated polypeptide chain comprising 116 amino acids, resulting in a molecular weight of 12.8 kDa. The purification of Procalcitonin is achieved through standard chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT17831
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.
Definition and Classification

Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, which is involved in calcium homeostasis. It is classified as a biomarker, particularly useful in the diagnosis and management of bacterial infections and sepsis. Procalcitonin is produced by the C-cells of the thyroid gland and by various other tissues in response to pro-inflammatory stimuli, especially bacterial infections.

Biological Properties

Key Biological Properties: Procalcitonin is a 116-amino acid peptide with a molecular weight of approximately 13 kDa. It is encoded by the CALC-1 gene and is initially synthesized as a larger precursor molecule, pre-procalcitonin, which is then cleaved to form procalcitonin.

Expression Patterns: Under normal physiological conditions, procalcitonin levels in the blood are very low. However, during bacterial infections, its expression is significantly upregulated, leading to elevated serum levels.

Tissue Distribution: Procalcitonin is primarily produced in the C-cells of the thyroid gland. However, during systemic infections, it can be produced by various other tissues, including the liver, lungs, and kidneys.

Biological Functions

Primary Biological Functions: Procalcitonin serves as a precursor to calcitonin, which plays a role in calcium and phosphate metabolism. However, its primary clinical significance lies in its role as a biomarker for bacterial infections and sepsis.

Role in Immune Responses: Procalcitonin is involved in the body’s immune response to bacterial infections. Its levels increase significantly in response to bacterial endotoxins and inflammatory cytokines, making it a valuable marker for detecting bacterial infections.

Pathogen Recognition: Procalcitonin is not directly involved in pathogen recognition but serves as an indicator of the presence of bacterial pathogens in the body. Elevated levels of procalcitonin suggest an ongoing bacterial infection, prompting further diagnostic and therapeutic measures.

Modes of Action

Mechanisms with Other Molecules and Cells: Procalcitonin interacts with various immune cells and molecules during bacterial infections. It is produced in response to pro-inflammatory cytokines such as IL-1β, TNF-α, and IL-6, which are released during bacterial infections.

Binding Partners: Procalcitonin itself does not have specific binding partners but is part of a complex network of inflammatory mediators that regulate the immune response to bacterial infections.

Downstream Signaling Cascades: The production of procalcitonin is part of the acute-phase response to bacterial infections. Its synthesis is upregulated by inflammatory cytokines, leading to increased serum levels, which can be measured to assess the severity of the infection.

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of procalcitonin is tightly regulated by inflammatory cytokines. During bacterial infections, cytokines such as IL-1β, TNF-α, and IL-6 stimulate the production of procalcitonin in various tissues.

Transcriptional Regulation: The CALC-1 gene, which encodes procalcitonin, is transcriptionally regulated by inflammatory signals. The promoter region of the CALC-1 gene contains binding sites for transcription factors that are activated during inflammation.

Post-Translational Modifications: Procalcitonin undergoes several post-translational modifications, including cleavage of the pre-procalcitonin precursor to form the mature procalcitonin peptide.

Applications

Biomedical Research: Procalcitonin is extensively studied in biomedical research for its role as a biomarker in bacterial infections and sepsis. Research focuses on understanding its regulation, expression patterns, and potential therapeutic applications.

Diagnostic Tools: Procalcitonin is widely used as a diagnostic marker for bacterial infections and sepsis. Elevated levels of procalcitonin in the blood indicate the presence of a bacterial infection, aiding in the diagnosis and management of patients.

Therapeutic Strategies: While procalcitonin itself is not used as a therapeutic agent, its role as a biomarker helps guide antibiotic therapy. Monitoring procalcitonin levels can help determine the effectiveness of treatment and guide decisions on the duration of antibiotic therapy.

Role in the Life Cycle

Development: Procalcitonin levels are generally low during normal development and in healthy individuals. Its expression is upregulated in response to bacterial infections, making it a valuable marker for detecting infections in individuals of all ages.

Aging: The role of procalcitonin in aging is not well understood. However, its utility as a biomarker for bacterial infections remains consistent across different age groups.

Disease: Procalcitonin plays a crucial role in the diagnosis and management of bacterial infections and sepsis. Elevated levels of procalcitonin are associated with severe bacterial infections, making it a valuable tool in clinical practice.

© Copyright 2024 Thebiotek. All Rights Reserved.