Product List

CXCL4 Variant 1 Human

Platelet Factor-4 Variant 1 Human Recombinant

CXCL4 Variant-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8.7 kDa.
The CXCL4 Variant-1 is fused to 6xHis tag at N-Terminus and purified by standard chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT22219
Source
Escherichia Coli.
Appearance
Sterile Filtered white lyophilized powder.

PF 4 Human

Platelet Factor-4 Human Recombinant (CXCL4)

CXCL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa.
Shipped with Ice Packs
Cat. No.
BT22293
Source
Escherichia Coli.
Appearance
Sterile Filtered white lyophilized powder.

PF 4 Mouse

Platelet Factor-4 Mouse Recombinant (CXCL4)

CXCL4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 76 amino acids and having a molecular mass of 8.2kDa.
Shipped with Ice Packs
Cat. No.
BT22372
Source
Escherichia Coli.
Appearance
Sterile Filtered white lyophilized powder.

PF 4 Protein

Platelet Factor-4 Human (CXCL4)

Human PF-4 a 7.8 kDa protein consisting of 70 amino acid residues.
Shipped with Ice Packs
Cat. No.
BT22418
Source
Human Platelets.
Appearance
Sterile Filtered white lyophilized powder.

PF4 Bovine

Platelet Factor-4 (CXCL4) Bovine Recombinant

Platelet Factor-4 (CXCL4) Bovine Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 88 amino acid and having a molecular mass of approximately 9.5kDa.
PF4 is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT22523
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

PF4 Human, His

Platelet Factor-4 Human Recombinant (CXCL4), His Tag

PF4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 91 amino acids (32-101 a.a) and having a molecular mass of 10kDa.
PF4 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22610
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless clear solution.

PF4V1 Human

Platelet Factor 4 Variant 1 Human Recombinant

PF4V1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (31-104 a.a.) and having a molecular mass of 10.6kDa.
PF4V1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22661
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless clear solution.

Introduction

Definition and Classification

Platelet Factor-4 (PF4), also known as CXCL4, is a small cytokine belonging to the CXC chemokine family . It is released from the alpha-granules of activated platelets during platelet aggregation and plays a crucial role in promoting blood coagulation by moderating the effects of heparin-like molecules .

Biological Properties

Key Biological Properties: PF4 is a 70-amino acid protein that binds with high affinity to heparin . It is chemotactic for neutrophils, fibroblasts, and monocytes .

Expression Patterns: PF4 is primarily produced by platelets and megakaryocytes . It is also expressed in monocytes .

Tissue Distribution: PF4 is stored in the alpha-granules of platelets and is released upon platelet activation .

Biological Functions

Primary Biological Functions: PF4 neutralizes heparin-like molecules on the endothelial surface of blood vessels, inhibiting local antithrombin activity and promoting coagulation . It also acts as a strong chemoattractant for neutrophils and fibroblasts, playing a role in inflammation and wound repair .

Role in Immune Responses: PF4 is involved in innate and adaptive immunity, particularly in events where platelets are activated in response to infections .

Pathogen Recognition: PF4 has been shown to kill malaria parasites within erythrocytes by selectively lysing the parasite’s digestive vacuole .

Modes of Action

Mechanisms with Other Molecules and Cells: PF4 interacts with heparin and other negatively charged proteoglycans . It also binds to the chemokine receptor CXCR3-B .

Binding Partners: PF4 forms complexes with heparin, which can lead to heparin-induced thrombocytopenia (HIT), an immune-mediated disorder .

Downstream Signaling Cascades: PF4 activates the c-Mpl–Jak2 pathway in platelets, resulting in JAK2-STAT3/5 signaling .

Regulatory Mechanisms

Transcriptional Regulation: PF4 expression is regulated at the transcriptional level in megakaryocytes and monocytes .

Post-Translational Modifications: PF4 undergoes post-translational modifications that affect its secretion and activity .

Applications

Biomedical Research: PF4 is used in research to study coagulation, inflammation, and immune responses .

Diagnostic Tools: PF4 levels can be measured to diagnose conditions like heparin-induced thrombocytopenia (HIT) and other thrombotic disorders .

Therapeutic Strategies: PF4 has potential therapeutic applications in treating thrombosis, inflammation, and certain infections .

Role in the Life Cycle

Development: PF4 plays a role in the regulation of coagulation processes during development .

Aging: PF4 levels and activity can influence age-related conditions, including cognitive decline and neuroinflammation .

Disease: PF4 is implicated in various diseases, including thrombosis, atherosclerosis, and inflammatory conditions .

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