Product List

SDF2 Human, sf9

Stromal Cell-Derived Factor 2, Sf9 Human Recombinant

SDF2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 202 amino acids (19-211a.a.) and having a molecular mass of 22.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SDF2 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT24232
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

SDF 1a Human

Stromal Cell-Derived Factor-1 Alpha Human Recombinant (CXCL12)

Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8004 Dalton.
The SDF-1a is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23211
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

SDF 1a Human, His

Stromal Cell-Derived Factor-1 alpha Human Recombinant, His Tag

Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 78 amino acids, having a molecular mass of 9.2 kDa.
The SDF-1a is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23301
Source
Escherichia Coli.
Appearance
White lyophilized (freeze-dried) powder.

SDF 1a Mouse

Stromal Cell-Derived Factor-1 Alpha Mouse Recombinant (CXCL12)

Stromal Cell-Derived Factor-1 alpha Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8 kDa.

The SDF-1a is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT23378
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

SDF 1a Mouse, His

Stromal Cell-Derived Factor-1 alpha (CXCL12), Mouse Recombinant, His Tag

SDF 1a Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 91 amino acids (22-89 a.a) and having a molecular mass of 10.4kDa. SDF 1a is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23468
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

SDF 1a Rat

Stromal Cell-Derived Factor-1 alpha Rat Recombinant (CXCL12)

Stromal Cell-Derived Factor-1 alpha Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 7.9 kDa.
The SDF-1a is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23539
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

SDF 1b Feline

Stromal Cell-Derived Factor-1 beta Feline Recombinant (CXCL12)

Stromal Cell-Derived Factor-1 beta Feline Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8526 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23622
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

SDF 1b Human

Stromal Cell Derived Factor-1 Beta Human Recombinant (CXCL12)

Stromal Cell-Derived Factor-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8508 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23682
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

SDF 1b Human, His

Stromal Cell-Derived Factor-1 beta Human Recombinant (CXCL12), His Tag

SDF-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 93 amino acids (22-93 a.a.) and having a molecular mass of 10.8 KDa.
The SDF-1b is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23751
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

SDF 1b Mouse

Stromal Cell Derived Factor-1 Beta Mouse Recombinant (CXCL12)

Stromal Cell-Derived Factor-1 beta Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8513 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23868
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Introduction

Definition and Classification

Stromal cell-derived factor 1 (SDF-1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein encoded by the CXCL12 gene on chromosome 10 in humans . It belongs to the CXC chemokine family, characterized by the presence of four conserved cysteines that form two disulfide bonds . SDF-1 exists in multiple isoforms, primarily SDF-1α (CXCL12a) and SDF-1β (CXCL12b), produced through alternative splicing .

Biological Properties

Key Biological Properties: SDF-1 is a potent chemotactic factor for lymphocytes and plays a crucial role in the migration and homing of hematopoietic stem cells . It is involved in various physiological processes, including embryogenesis, hematopoiesis, and angiogenesis .

Expression Patterns: SDF-1 is ubiquitously expressed in many tissues and cell types . It is particularly abundant in the brain, thymus, heart, lung, liver, kidney, spleen, platelets, and bone marrow .

Tissue Distribution: During embryogenesis, SDF-1 directs the migration of hematopoietic cells from the fetal liver to the bone marrow and the formation of large blood vessels . In adults, it is constitutively expressed in several organs, including the bone marrow, skin, heart, and brain endothelium .

Biological Functions

Primary Biological Functions: SDF-1 is essential for the retention of hematopoietic progenitor and stem cells in the bone marrow . It also plays a critical role in the development of the cardiovascular and nervous systems .

Role in Immune Responses: SDF-1 is strongly chemotactic for lymphocytes, guiding their migration to sites of inflammation . It also influences the differentiation and function of monocytes and macrophages .

Pathogen Recognition: SDF-1 has been implicated in the immune response to various pathogens by modulating the activity of immune cells .

Modes of Action

Mechanisms with Other Molecules and Cells: SDF-1 exerts its effects primarily through binding to its receptors, CXCR4 and CXCR7 . CXCR4 is a classic G-protein-coupled receptor that mediates signal transduction, while CXCR7 acts as a scavenger receptor modulating CXCR4 function .

Binding Partners: SDF-1 interacts with glycosaminoglycans (GAGs) in tissues and on the endothelium, facilitating its presentation to passing leukocytes .

Downstream Signaling Cascades: Upon binding to CXCR4, SDF-1 activates several intracellular signaling pathways, including the PI3K and ERK/MAPK pathways, leading to chemotaxis, cell survival, and proliferation .

Regulatory Mechanisms

Transcriptional Regulation: The expression of SDF-1 is regulated at the transcriptional level by various factors, including hypoxia and inflammatory cytokines .

Post-Translational Modifications: SDF-1 activity is modulated by post-translational modifications such as proteolytic cleavage, citrullination, and nitration . These modifications can alter its chemotactic activity and receptor binding affinity .

Applications

Biomedical Research: SDF-1 is widely studied for its role in stem cell biology, cancer metastasis, and tissue regeneration .

Diagnostic Tools: Elevated levels of SDF-1 have been associated with various diseases, including cancer and cardiovascular diseases, making it a potential biomarker for diagnosis .

Therapeutic Strategies: Targeting the SDF-1/CXCR4 axis has shown promise in cancer therapy, as it can inhibit tumor growth and metastasis . Additionally, SDF-1 has potential applications in enhancing wound healing and tissue repair .

Role in the Life Cycle

Development: SDF-1 is crucial for the proper development of the cardiovascular and nervous systems during embryogenesis .

Aging and Disease: In adults, SDF-1 plays a role in maintaining tissue homeostasis and regulating immune responses . Dysregulation of the SDF-1/CXCR4 axis has been implicated in various age-related diseases, including cancer and cardiovascular diseases .

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