Cytokines
Product List
IL12 Human, Sf9
Interleukin-12 Human Recombinant, Sf9
Baculovirus.
IL12 Mouse, Sf9
Interleukin 12, Sf9 Human Recombinant
Recombinant mouse IL12, produced using a baculovirus expression system in Sf9 insect cells, is a glycosylated heterodimer linked by disulfide bonds (formed through cysteine residues, indicated in bold). It consists of IL12A (amino acids 23-335, total 319 aa, molecular weight 35.7 kDa) and IL12B (amino acids 23-215, total 199 aa, molecular weight 22.5 kDa), resulting in a predicted total molecular mass of 58.3 kDa. However, it's important to note that the apparent molecular weight on SDS-PAGE might be higher. IL12A is tagged with a 6-amino acid His-tag at its C-terminus. The protein is purified using proprietary chromatographic techniques.
Sf9, Baculovirus cells.
IL12RB1 Human
Interleukin 12 Receptor Beta 1 Human Recombinant
IL12RB1 Human, Sf9
Interleukin 12 Receptor Beta 1 Human Recombinant, Sf9
IL17F Human, sf9
Interleukin 17F Human Recombinant, sf9
IL17F, produced using Sf9 insect cells infected with a baculovirus expression system, is a single-chain polypeptide that has undergone glycosylation. It consists of 142 amino acids (specifically, amino acids 31 to 163) and has a molecular mass of 16 kDa. When analyzed using SDS-PAGE, it appears as a band in the range of approximately 18-28 kDa. This IL17F protein is engineered with a 9-amino acid Histidine tag located at the C-terminus to facilitate purification. The protein is purified using specialized chromatographic methods.
IL17F Mouse
Interleukin-17F Mouse Recombinant
IL17F Rat
Interleukin-17F Rat Recombinant
IL17RA Human
Interleukin-17 Receptor Alpha Human Recombinant
Sf9, Baculovirus cells.
IL1F10 Human
Interleukin 1 Family, Member 10 Human Recombinant
IL1F10 Human His
Interleukin 1 Family, Member 10 Human Recombinant, His Tag
Introduction
Interleukins (ILs) are a group of cytokines, which are secreted proteins and signal molecules primarily produced by leukocytes (white blood cells). They play a crucial role in the immune system by mediating communication between cells . The human genome encodes more than 50 interleukins and related proteins . Interleukins are classified based on their structure and function, with common families including IL-1, IL-2, IL-6, and IL-12 .
Key Biological Properties: Interleukins are biologically active glycoproteins derived primarily from activated lymphocytes and macrophages . They induce T-lymphocyte activation and proliferation, augment neutrophil, macrophage, and T-lymphocyte cytotoxicity, and promote B lymphocyte and multilineage bone marrow stem-cell precursor growth and differentiation .
Expression Patterns and Tissue Distribution: Interleukins are produced by various cell types, including immune cells like macrophages and lymphocytes . They are expressed in different tissues, such as the bone marrow, thymus, and other epithelial cells .
Primary Biological Functions: Interleukins modulate the growth, differentiation, and activation of immune cells during inflammatory and immune responses . They play a role in both innate and adaptive immune responses .
Role in Immune Responses and Pathogen Recognition: Interleukins are involved in the regulation of immune responses, inflammatory reactions, and hematopoiesis . They help coordinate the body’s response to infections, inflammation, and other immune challenges .
Mechanisms with Other Molecules and Cells: Interleukins interact with specific cell surface receptors to stimulate target cells . For example, IL-6 controls leucocyte recruitment, determines the activity and maintenance of the inflammatory infiltrate, and drives various innate and adaptive immune responses .
Binding Partners and Downstream Signaling Cascades: Interleukins bind to their respective receptors, triggering downstream signaling pathways such as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathways . These signaling cascades lead to various cellular responses, including proliferation, differentiation, and cytokine production .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of interleukins are regulated by complex molecular mechanisms. For example, IL-10 production is regulated by metabolic pathways and molecular signals downstream of the IL-10 receptor .
Transcriptional Regulation and Post-Translational Modifications: Interleukins undergo transcriptional regulation and post-translational modifications to ensure proper function. These modifications can include phosphorylation, glycosylation, and cleavage .
Biomedical Research: Interleukins are extensively studied in biomedical research for their roles in immune regulation and disease pathogenesis .
Diagnostic Tools: Interleukins serve as biomarkers for various diseases, aiding in diagnosis and monitoring of disease progression .
Therapeutic Strategies: Interleukins are used in therapeutic strategies, such as cancer immunotherapy and treatment of autoimmune diseases . For example, engineered cytokines from the IL-2 family have shown significant effects in tumor immunotherapy .
Role Throughout the Life Cycle: Interleukins play essential roles throughout the life cycle, from development to aging and disease. They regulate hematopoiesis, immune responses, and inflammation . For instance, IL-5 regulates eosinophil proliferation and differentiation, while IL-1 is involved in inflammatory responses .
