Product List

CTGF (182-250 a.a.) Human

Connective Tissue Growth Factor Human Recombinant (182-250 a.a.)

The Connective Tissue Growth Factor amino acids 182-250, produced in E.Coli, is a fusion protein with His Tag (4 kDa), having a total molecular mass of 15 kDa.
Shipped with Ice Packs
Cat. No.
BT2352
Source
Escherichia Coli.
Appearance
Sterile Filtered white lyophilized powder.

CTGF Human

Connective Tissue Growth Factor Human Recombinant

CTGF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and having a molecular mass of 11.2 kDa.
The CTGF is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2465
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

CTGF Human (183-255)

Connective Tissue Growth Factor (183-255 a.a.) Human Recombinant

CTGF Human Recombinant is a single, glycosylated polypeptide chain containing 80 amino acids (183-255a.a) and having a molecular mass of 9.1kDa (calculated). CTGF is fused to a 7 a.a His tag at N-terminal.

Shipped with Ice Packs
Cat. No.
BT2586
Source

HEK293 cells.

Appearance
Filtered White lyophilized (freeze-dried) powder.

CTGF Human, HEK

Connective Tissue Growth Factor Human Recombinant , HEK

The CTGF Human Recombinant produced in HEK293 cells, is 36kDa protein containing a total of 329 amino acid residues (aa 27-349) including a C-terminal 6×His tag.
Shipped with Ice Packs
Cat. No.
BT2676
Source
HEK293 cells.
Appearance
Filtered colorless solution.

CTGF Human, His

Connective Tissue Growth Factor Human Recombinant, His Tag

CTGF Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 344 amino acids (27-349) and having a molecular mass of 37.7kDa.
The CTGF is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2745
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Connective Tissue Growth Factor (CTGF), also known as cellular communication network factor 2 (CCN2), is a matricellular protein belonging to the CCN family of extracellular matrix-associated heparin-binding proteins . It plays a crucial role in various biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair .

Biological Properties

Key Biological Properties: CTGF is a cysteine-rich protein with four conserved domains: the insulin-like growth factor binding protein (IGFBP) domain, the von Willebrand type C repeats (vWC) domain, the thrombospondin type 1 repeat (TSR) domain, and a C-terminal domain (CT) with a cysteine knot motif .

Expression Patterns: CTGF is expressed in various tissues, including connective tissues, skin, and organs such as the liver and kidneys . Its expression is crucial during embryonic development and is also detected in several adulthood diseases .

Tissue Distribution: CTGF is widely distributed in tissues involved in wound healing and fibrotic processes, such as the skin, liver, kidneys, and lungs .

Biological Functions

Primary Biological Functions: CTGF is involved in cell adhesion, migration, proliferation, angiogenesis, and tissue repair . It plays a significant role in fibrotic diseases and various forms of cancer .

Role in Immune Responses and Pathogen Recognition: CTGF contributes to immune responses by modulating the activity of immune cells and influencing the extracellular matrix, which can affect pathogen recognition and response .

Modes of Action

Mechanisms with Other Molecules and Cells: CTGF exerts its functions by binding to various cell surface receptors, including integrin receptors, heparan sulfate proteoglycans (HSPGs), low-density lipoprotein receptor-related proteins (LRPs), and tropomyosin receptor kinase A (TrkA) . It also interacts with growth factors and extracellular matrix proteins .

Binding Partners and Downstream Signaling Cascades: CTGF binds to aggrecan, vascular endothelial growth factor (VEGF), and members of the transforming growth factor-beta (TGF-β) superfamily . These interactions activate downstream signaling cascades that regulate cell proliferation, migration, and differentiation .

Regulatory Mechanisms

Transcriptional Regulation: The expression of CTGF is regulated by various transcription factors and signaling pathways, including TGF-β, which can induce CTGF expression .

Post-Translational Modifications: CTGF undergoes post-translational modifications, such as phosphorylation and glycosylation, which can influence its activity and interactions with other molecules .

Applications

Biomedical Research: CTGF is a valuable target in biomedical research due to its involvement in fibrosis, cancer, and tissue repair .

Diagnostic Tools: Elevated levels of CTGF can serve as biomarkers for fibrotic diseases and certain cancers .

Therapeutic Strategies: Targeting CTGF with specific inhibitors or monoclonal antibodies has shown promise in treating fibrotic diseases and cancer .

Role in the Life Cycle

Development: CTGF is essential for embryonic development, particularly in skeletal development and angiogenesis . Knockout mice with disrupted CTGF genes exhibit severe developmental defects and perinatal lethality .

Aging and Disease: In adulthood, CTGF is associated with wound healing and fibrotic diseases . Its overexpression can lead to fibrosis in various organs, including the skin, kidneys, and lungs . CTGF also plays a role in cancer progression and metastasis .

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