CTGF Human (183-255)

Connective Tissue Growth Factor (183-255 a.a.) Human Recombinant

Recombinant Human CTGF is a single, glycosylated polypeptide chain consisting of 80 amino acids (183-255a.a). With a molecular weight of 9.1kDa (calculated), it includes a 7 a.a His tag at the N-terminal.

Shipped with Ice Packs
Cat. No.
BT2586
Source

HEK293 cells.

Appearance
White, lyophilized powder, filtered for purity.

CTGF Human, HEK

Connective Tissue Growth Factor Human Recombinant , HEK

CTGF Human Recombinant, produced in HEK293 cells, is a 36kDa protein comprising 329 amino acids (residues 27-349), including a C-terminal 6xHis tag.
Shipped with Ice Packs
Cat. No.
BT2676
Source
HEK293 cells.
Appearance
A clear, colorless liquid after filtration.

CTGF Human, His

Connective Tissue Growth Factor Human Recombinant, His Tag

Recombinant Human CTGF, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 344 amino acids (27-349) with a molecular weight of 37.7kDa. This protein includes a 21 amino acid His-Tag at the N-terminus and is purified using specialized chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2745
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

CTGF (182-250 a.a.) Human

Connective Tissue Growth Factor Human Recombinant (182-250 a.a.)

The Connective Tissue Growth Factor (CTGF) fragment encompassing amino acids 182 to 250, produced in E. coli bacteria, is a fusion protein. This means it's linked to a His Tag (a sequence of histidine amino acids), adding 4 kDa to its size, resulting in a total molecular weight of 15 kDa.
Shipped with Ice Packs
Cat. No.
BT2352
Source
Escherichia Coli.
Appearance
Sterile Filtered white lyophilized powder.

CTGF Human

Connective Tissue Growth Factor Human Recombinant

Recombinant human CTGF, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 98 amino acids. It has a molecular weight of 11.2 kDa and is purified using specialized chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT2465
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.
Definition and Classification

Connective Tissue Growth Factor (CTGF), also known as cellular communication network factor 2 (CCN2), is a matricellular protein belonging to the CCN family of extracellular matrix-associated heparin-binding proteins . It plays a crucial role in various biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair .

Biological Properties

Key Biological Properties: CTGF is a cysteine-rich protein with four conserved domains: the insulin-like growth factor binding protein (IGFBP) domain, the von Willebrand type C repeats (vWC) domain, the thrombospondin type 1 repeat (TSR) domain, and a C-terminal domain (CT) with a cysteine knot motif .

Expression Patterns: CTGF is expressed in various tissues, including connective tissues, skin, and organs such as the liver and kidneys . Its expression is crucial during embryonic development and is also detected in several adulthood diseases .

Tissue Distribution: CTGF is widely distributed in tissues involved in wound healing and fibrotic processes, such as the skin, liver, kidneys, and lungs .

Biological Functions

Primary Biological Functions: CTGF is involved in cell adhesion, migration, proliferation, angiogenesis, and tissue repair . It plays a significant role in fibrotic diseases and various forms of cancer .

Role in Immune Responses and Pathogen Recognition: CTGF contributes to immune responses by modulating the activity of immune cells and influencing the extracellular matrix, which can affect pathogen recognition and response .

Modes of Action

Mechanisms with Other Molecules and Cells: CTGF exerts its functions by binding to various cell surface receptors, including integrin receptors, heparan sulfate proteoglycans (HSPGs), low-density lipoprotein receptor-related proteins (LRPs), and tropomyosin receptor kinase A (TrkA) . It also interacts with growth factors and extracellular matrix proteins .

Binding Partners and Downstream Signaling Cascades: CTGF binds to aggrecan, vascular endothelial growth factor (VEGF), and members of the transforming growth factor-beta (TGF-β) superfamily . These interactions activate downstream signaling cascades that regulate cell proliferation, migration, and differentiation .

Regulatory Mechanisms

Transcriptional Regulation: The expression of CTGF is regulated by various transcription factors and signaling pathways, including TGF-β, which can induce CTGF expression .

Post-Translational Modifications: CTGF undergoes post-translational modifications, such as phosphorylation and glycosylation, which can influence its activity and interactions with other molecules .

Applications

Biomedical Research: CTGF is a valuable target in biomedical research due to its involvement in fibrosis, cancer, and tissue repair .

Diagnostic Tools: Elevated levels of CTGF can serve as biomarkers for fibrotic diseases and certain cancers .

Therapeutic Strategies: Targeting CTGF with specific inhibitors or monoclonal antibodies has shown promise in treating fibrotic diseases and cancer .

Role in the Life Cycle

Development: CTGF is essential for embryonic development, particularly in skeletal development and angiogenesis . Knockout mice with disrupted CTGF genes exhibit severe developmental defects and perinatal lethality .

Aging and Disease: In adulthood, CTGF is associated with wound healing and fibrotic diseases . Its overexpression can lead to fibrosis in various organs, including the skin, kidneys, and lungs . CTGF also plays a role in cancer progression and metastasis .

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