SPP1 Human, HEK

Osteopontin Human Recombinant, HEK

This product contains human Osteopontin protein produced in a lab setting using HEK293 cells. It is a highly purified, full-length version of the protein with a tag for easy detection and purification. The protein may appear larger than its calculated size on some laboratory tests due to the addition of sugar molecules.
Shipped with Ice Packs
Cat. No.
BT25688
Source
HEK293 cells.
Appearance
The product is provided as a white powder that has been sterilized and freeze-dried.

SPP1 Mouse

Osteopontin 1 Mouse Recombinant

Recombinant SPP1 Mouse, produced in HEK293 cells, is a single-chain glycosylated polypeptide. This protein comprises 287 amino acids (17-294 a.a), has a molecular weight of 31.8kDa, and includes a C-terminal 6-amino acid His tag. Purification is achieved through proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT25781
Source

HEK293 Cells.

Appearance
A clear, colorless solution that is sterile-filtered.

SPP1 Human

Osteopontin Human Recombinant

This product consists of a single, non-glycosylated polypeptide chain of human Secreted Phosphoprotein-1 (SPP1), recombinantly produced in E. coli. The protein encompasses amino acids 17-314, representing a fragment of the full SPP1 sequence. It has a molecular weight of 36.2 kDa, although this may appear higher on SDS-PAGE due to the presence of a 20 amino acid His-Tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT25506
Source
Escherichia Coli.
Appearance
The product appears as a clear, colorless solution that has been sterilized by filtration.

SPP1 Human, Active

Osteopontin Human Recombinant, BioActive

Recombinant Human SPP1, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing 321 amino acids (residues 17-314). With a molecular weight of 36.2 kDa, this protein includes a 23 amino acid His tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT25603
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a multifunctional glycoprotein encoded by the SPP1 gene. It belongs to the SIBLING (Small Integrin-Binding Ligand, N-linked Glycoprotein) family of proteins . OPN was initially identified in osteoblasts and is highly expressed in bone, but it is also found in various other tissues .

Biological Properties

Key Biological Properties: OPN is a phosphorylated and glycosylated protein that undergoes extensive post-translational modifications . It has an arginine-glycine-aspartic acid (RGD) cell-binding sequence, a calcium-binding site, and two heparin-binding domains .

Expression Patterns: OPN is expressed by a variety of cell types, including osteoblasts, osteocytes, macrophages, endothelial cells, smooth muscle cells, and epithelial cells .

Tissue Distribution: OPN is found in almost every tissue in the body, including bone, skin, brain, body fluids, cartilage, and various tumors .

Biological Functions

Primary Biological Functions: OPN plays a critical role in bone remodeling, biomineralization, and wound healing . It mediates cell adhesion, migration, and survival through interactions with integrins and CD44 receptors .

Role in Immune Responses: OPN functions as a Th1 cytokine, promoting cell-mediated immune responses and playing a role in chronic inflammatory and autoimmune diseases . It is involved in leukocyte recruitment and activation during immune responses .

Pathogen Recognition: OPN is associated with many inflammatory conditions caused by infections, allergic responses, autoimmunity, and tissue damage .

Modes of Action

Mechanisms with Other Molecules and Cells: OPN interacts with multiple integrin receptors, including the vitronectin receptor (αvβ3) and various β1 and β5 integrins . It also binds to CD44 isoforms via multiple sites .

Binding Partners: OPN binds to integrins and CD44 receptors, initiating downstream signaling cascades . It can be cleaved by thrombin, exposing additional cryptic binding sites and producing functional chemotactic fragments .

Downstream Signaling Cascades: The binding of OPN to its receptors activates several downstream signaling pathways, including PI3K/AKT, FAK/AKT, and NF-κB, leading to cell proliferation, migration, and inflammation .

Regulatory Mechanisms

Transcriptional Regulation: The regulation of OPN expression involves various motifs in its promoter, including purine-rich sequences, ets-like sequences, glucocorticoid and vitamin D response elements, and interferon-inducible elements . Pro-inflammatory cytokines such as TNF-α and IL-1β strongly induce OPN expression .

Post-Translational Modifications: OPN undergoes extensive post-translational modifications, including phosphorylation, glycosylation, and cleavage . These modifications affect its function and interactions with other molecules .

Applications

Biomedical Research: OPN is widely studied in the context of bone remodeling, cancer, and immune responses . It serves as a biomarker for various diseases, including cancer and chronic inflammatory conditions .

Diagnostic Tools: OPN levels in plasma and serum are used as diagnostic markers for several diseases, including cancer and cardiovascular diseases .

Therapeutic Strategies: Targeting OPN and its signaling pathways is being explored as a therapeutic strategy for cancer, autoimmune diseases, and chronic inflammatory conditions .

Role in the Life Cycle

Development: OPN is involved in implantation and placentation, playing a crucial role in the uterine-placental microenvironment during pregnancy .

Aging and Disease: OPN contributes to tissue maintenance, wound healing, and immune regulation throughout life . It is implicated in the pathogenesis of several conditions, including atherosclerosis, cancer, autoimmune disorders, chronic inflammation, and sepsis .

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