SCF Canine

Stem Cell Factor Canine Recombinant

Recombinant Canine Stem Cell Factor, produced in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 18.4 kDa. It comprises 165 amino acids. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT1926
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.

SCF Human

Stem Cell Factor Human Recombinant

Recombinant Human Stem Cell Factor, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 165 amino acids. It has a molecular mass of 18.4 kDa. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2067
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.

SCF Human, HEK

Stem Cell Factor Human Recombinant, HEK

Recombinant human SCF, produced in HEK cells, is a glycosylated monomer. Due to glycosylation, its molecular weight ranges from 35 to 45 kDa. The purification of SCF is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT2156
Source
HEK.
Appearance
Sterile Filtered White lyophilized powder.

SCF Human, Sf9

Stem Cell Factor Human Recombinant, Sf9

Recombinant Human Stem Cell Factor, expressed in insect cells, is a single, glycosylated polypeptide chain with 165 amino acids and a molecular weight of 18.4 kDa. This SCF protein has a C-terminal His-tag (6xHis) and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2247
Source
Sf9, Insect Cells.
Appearance
Sterile, white lyophilized (freeze-dried) powder.

SCF Mouse

Stem Cell Factor Mouse Recombinant

Recombinant Mouse Stem Cell Factor, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 165 amino acids. With a molecular mass of 18.3 kDa, this purified protein is obtained through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2336
Source
Escherichia Coli.
Appearance
Sterile, white lyophilized powder.

SCF Rat

Stem Cell Factor Rat Recombinant

Recombinant Rat Stem cell factor, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 164 amino acids (26-189) with a molecular weight of 18.4 kDa. The purification of Rat SCF is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2436
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Definition and Classification

Stem Cell Factor (SCF), also known as KIT ligand, steel factor, or mast cell growth factor, is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein . It plays a crucial role in hematopoiesis, spermatogenesis, and melanogenesis .

Biological Properties

Key Biological Properties: SCF is a growth factor that promotes the proliferation, migration, survival, and differentiation of hematopoietic progenitors, melanocytes, and germ cells . It is expressed by fibroblasts and endothelial cells throughout the body .

Expression Patterns and Tissue Distribution: SCF is expressed in various tissues, including the visceral pleura, cardia, lower lobe of the lung, pylorus, lateral nuclear group of the thalamus, middle temporal gyrus, parietal pleura, endothelial cells, skin of the hip, and seminal vesicles .

Biological Functions

Primary Biological Functions: SCF plays a vital role in hematopoiesis (formation of blood cells), spermatogenesis (formation of sperm cells), and melanogenesis (formation of melanin) . It is essential for the survival and development of hematopoietic stem cells, melanocytes, and germ cells .

Role in Immune Responses and Pathogen Recognition: SCF is involved in the regulation of immune responses by promoting the proliferation and differentiation of immune cells . It also plays a role in pathogen recognition by enhancing the function of immune cells .

Modes of Action

Mechanisms with Other Molecules and Cells: SCF binds to the c-KIT receptor, causing the receptor to homodimerize and auto-phosphorylate at tyrosine residues . This interaction activates multiple downstream signaling pathways, including the phosphatidylinositol-3-kinase (PI3K), Src family members, the JAK/STAT pathway, and the Ras–Raf–MAP kinase cascade .

Binding Partners and Downstream Signaling Cascades: The binding of SCF to c-KIT activates various signaling cascades that promote cell survival, proliferation, and differentiation . These pathways include the PI3K/Akt pathway, which is involved in cell survival, and the MAPK pathway, which is involved in cell proliferation .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of SCF are regulated by various transcription factors and signaling pathways . Transcriptional regulation involves the activation of specific genes that encode SCF, while post-translational modifications, such as phosphorylation, can modulate the activity of SCF .

Transcriptional Regulation and Post-Translational Modifications: Transcription factors, such as PU.1, play a crucial role in the regulation of SCF expression . Post-translational modifications, including phosphorylation and glycosylation, can affect the stability and activity of SCF .

Applications

Biomedical Research: SCF is widely used in biomedical research to study hematopoiesis, stem cell biology, and immune responses . It is also used to culture and expand hematopoietic stem cells in vitro .

Diagnostic Tools: SCF can be used as a biomarker for certain diseases, such as mastocytosis and certain types of leukemia . Its levels can be measured to diagnose and monitor these conditions .

Therapeutic Strategies: SCF has potential therapeutic applications in regenerative medicine and stem cell therapy . It can be used to enhance the engraftment and survival of transplanted stem cells .

Role in the Life Cycle

Role Throughout the Life Cycle: SCF plays a critical role throughout the life cycle, from development to aging and disease . During development, SCF is essential for the formation of blood cells, germ cells, and melanocytes . In adulthood, SCF continues to support the maintenance and function of these cells .

From Development to Aging and Disease: SCF is involved in various physiological processes, including tissue repair and regeneration . Dysregulation of SCF signaling can lead to various diseases, such as anemia, infertility, and pigmentation disorders .

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