Product List

HGF (32-285) Human

Hepatoma-Derived Growth Factor (32-285 a.a) Human Recombinant

HGF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (32-285 a.a.) and having a molecular mass of 29.8kDa. HGF is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15613
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

HGF B Human

Hepatocyte Growth Factor B Chain Human Recombinant

HGF-B Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 234 amino acids fragment (495-728) having a molecular weight of 34kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The HGF-B is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15683
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

HGF Human

Hepatocyte Growth Factor Human Recombinant

Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain containing 692 a.a and having a total molecular mass of 78.0 KDa.
The HGF is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT15776
Source
Insect cells.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

HGF Human, CHO

Hepatocyte Growth Factor Human Recombinant, CHO

Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of approximately 75kDa.
The HGF is purified by proprietary chromatographic techniques. 

Shipped with Ice Packs
Cat. No.
BT15869
Source
Chinese Hamster Ovarian Cells.
Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

HGF Human, HEK

Hepatocyte Growth Factor Human Recombinant, HEK

HGF Human Recombinant produced in HEK cells is a glycosylated disulfide-linked heterodimer, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 80kDa.

The HGF is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT15945
Source
HEK.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

HGF Mouse

Hepatocyte Growth Factor Mouse Recombinant

HGF Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 1146 amino acids (25-931aa) and having a molecular mass of 127.8kDa. HGF is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT16021
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered colorless solution.

pHGF Porcine

Hepatocyte promoting Growth Factor Porcine

Hepatocyte Growth Factor porcine is extracted from pig liver.
The HGF is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16117
Source
Pig Liver.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Introduction

Definition and Classification

Hepatocyte Growth Factor (HGF) is a multifunctional protein that plays a critical role in various biological processes. It is classified as a growth factor and is also known as scatter factor (SF). HGF is a member of the plasminogen-related growth factor family and is primarily produced by mesenchymal cells.

Biological Properties

Key Biological Properties: HGF is a heterodimeric molecule composed of an alpha-chain and a beta-chain linked by a disulfide bond. It is secreted as an inactive precursor (pro-HGF) and is activated by proteolytic cleavage.

Expression Patterns: HGF is expressed in various tissues, including the liver, kidneys, lungs, and the nervous system. Its expression is regulated by various factors, including cytokines, growth factors, and hormones.

Tissue Distribution: HGF is widely distributed in the body, with high concentrations found in the liver, where it plays a crucial role in liver regeneration and repair. It is also present in other tissues such as the kidneys, lungs, and the nervous system.

Biological Functions

Primary Biological Functions: HGF is involved in a wide range of biological functions, including cell proliferation, differentiation, motility, and survival. It is essential for embryonic development, tissue regeneration, and wound healing.

Role in Immune Responses: HGF modulates immune responses by influencing the behavior of various immune cells, including macrophages, dendritic cells, and T cells. It has anti-inflammatory properties and can promote tissue repair and regeneration in response to injury.

Pathogen Recognition: HGF can enhance the ability of immune cells to recognize and respond to pathogens, thereby contributing to the body’s defense mechanisms.

Modes of Action

Mechanisms with Other Molecules and Cells: HGF exerts its effects by binding to its receptor, c-Met, a tyrosine kinase receptor. This binding triggers a cascade of downstream signaling events that mediate various cellular responses.

Binding Partners: HGF interacts with several binding partners, including heparan sulfate proteoglycans, which facilitate its binding to the c-Met receptor and enhance its biological activity.

Downstream Signaling Cascades: Upon binding to c-Met, HGF activates multiple signaling pathways, including the PI3K/Akt, MAPK/ERK, and STAT pathways. These pathways regulate various cellular processes, such as proliferation, survival, and migration.

Regulatory Mechanisms

Regulatory Mechanisms that Control Expression and Activity: The expression and activity of HGF are tightly regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational mechanisms.

Transcriptional Regulation: HGF gene expression is regulated by various transcription factors, including NF-κB, AP-1, and STAT3. These factors can be activated by cytokines, growth factors, and other signaling molecules.

Post-Translational Modifications: HGF undergoes several post-translational modifications, including proteolytic cleavage, glycosylation, and phosphorylation. These modifications are essential for its activation, stability, and biological activity.

Applications

Biomedical Research: HGF is widely studied in biomedical research due to its diverse biological functions and therapeutic potential. It is used as a model to study cell signaling, tissue regeneration, and cancer biology.

Diagnostic Tools: HGF levels can serve as biomarkers for various diseases, including liver disease, kidney disease, and cancer. Measuring HGF levels in biological samples can aid in the diagnosis and prognosis of these conditions.

Therapeutic Strategies: HGF has therapeutic potential in regenerative medicine and tissue engineering. It is being explored as a treatment for liver cirrhosis, myocardial infarction, and chronic kidney disease. Additionally, HGF-based therapies are being developed for cancer treatment due to its ability to inhibit tumor growth and metastasis.

Role in the Life Cycle

Development: HGF plays a crucial role in embryonic development, particularly in the formation of the liver, kidneys, and nervous system. It regulates cell proliferation, differentiation, and migration during organogenesis.

Aging: HGF levels decline with age, which may contribute to the reduced regenerative capacity of tissues in older individuals. Enhancing HGF activity has been proposed as a potential strategy to promote healthy aging and tissue repair.

Disease: Dysregulation of HGF signaling is associated with various diseases, including cancer, fibrosis, and inflammatory conditions. Understanding the role of HGF in these diseases can provide insights into their pathogenesis and lead to the development of novel therapeutic approaches.

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