OPG Human, His

Osteoprotegerin Human Recombinant, His Tag

Recombinant Human OCIF, a 31 kDa protein encompassing amino acids 201-401, is produced in E.coli cells. This non-glycosylated polypeptide chain includes a 4 kDa His tag and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26220
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

OPG Mouse

Osteoprotegerin Mouse Recombinant

Recombinant Mouse OPG, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 388 amino acids (22-401a.a.). It has a molecular mass of 44.4kDa, but on SDS-PAGE, the molecular size appears to be approximately 40-57kDa. This OPG protein is expressed with an 8 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26302
Source
Sf9, Baculovirus cells.
Appearance
A sterile, filtered solution that is colorless.

OPG Human, HEK

Osteoprotegerin Human Recombinant, HEK

Recombinant Human OPG is a single, glycosylated polypeptide chain comprising 393 amino acids (22-401a.a). It has a molecular mass of 45.0kDa (calculated) and is fused to a 13 a.a FLAG-tag at the N-terminal.
Shipped with Ice Packs
Cat. No.
BT26026
Source

HEK293 Cells.

Appearance
White, sterile, lyophilized (freeze-dried) powder.

OPG Human, Hi-5

Osteoprotegerin Human Recombinant, Hi-5

Recombinant Osteoprotegerin, expressed in baculovirus, is a single, glycosylated polypeptide chain consisting of 398 amino acid residues (22-401 a.a.). It has a calculated molecular mass of 45.6 kDa. This OPG protein is engineered with a 17 amino acid His tag at the C-terminus to facilitate purification, which is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26132
Source
Hi-5.
Appearance
The product is a sterile, filtered solution that is colorless and clear.

OPG Fc Human

Osteoprotegerin Human Recombinant /Fc Chimera

Recombinant OPG, a laboratory-produced version of the naturally occurring protein, is often used in research and therapeutic applications. This particular recombinant OPG is a fusion protein consisting of the extracellular domain of human OPG linked to the Fc region of human IgG1. It has a molecular weight of approximately 109.6 kDa, as determined by its amino acid sequence.
Shipped with Ice Packs
Cat. No.
BT25850
Source
Pichia Pastoris.
Appearance
The product is provided as a white powder that has been sterilized by filtration and lyophilized (freeze-dried).

OPG Human

Osteoprotegerin Human Recombinant

Recombinant Human Osteoprotegerin is a laboratory-produced protein that mimics the structure and function of naturally occurring human OPG. This protein is produced using E. coli bacteria as a host organism and undergoes a purification process to ensure its high purity and biological activity. It is a single, non-glycosylated polypeptide chain composed of 174 amino acids, resulting in a molecular weight of 20kDa.
Shipped with Ice Packs
Cat. No.
BT25948
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder
Definition and Classification

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) or tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a cytokine receptor belonging to the tumor necrosis factor (TNF) receptor superfamily . It is encoded by the TNFRSF11B gene and functions primarily as a decoy receptor for receptor activator of nuclear factor kappa-B ligand (RANKL), inhibiting osteoclastogenesis and bone resorption .

Biological Properties

Key Biological Properties: OPG is a glycoprotein that exists as a homodimer linked by disulfide bonds. It has multiple glycosylation sites, resulting in a mature secreted form of 110-120 kDa .

Expression Patterns: OPG is expressed in various tissues, including cartilage, aorta, endometrium, thyroid gland, and arteries . It is also expressed in B cells and dendritic cells, regulated by CD40 .

Tissue Distribution: OPG is found in high concentrations in the bone, where it plays a crucial role in bone metabolism. It is also present in the cardiovascular system, endocrine tissues, and immune cells .

Biological Functions

Primary Biological Functions: OPG primarily functions to inhibit osteoclast differentiation and activation by binding to RANKL, preventing it from interacting with its receptor RANK . This action is essential for maintaining bone density and preventing excessive bone resorption .

Role in Immune Responses and Pathogen Recognition: OPG influences immune responses by regulating B cell development and dendritic cell function. It also binds to TNF-related apoptosis-inducing ligand (TRAIL), inhibiting TRAIL-induced apoptosis in specific cells, including tumor cells .

Modes of Action

Mechanisms with Other Molecules and Cells: OPG acts as a decoy receptor for RANKL, binding to it and preventing its interaction with RANK. This inhibition blocks the signaling pathways that lead to osteoclast differentiation and activation . OPG also binds to TRAIL, syndecan-1, glycosaminoglycans, von Willebrand factor, and factor VIII-von Willebrand factor complex .

Binding Partners and Downstream Signaling Cascades: By binding to RANKL, OPG inhibits the RANKL-RANK interaction, which is crucial for osteoclastogenesis. This inhibition prevents the activation of downstream signaling cascades involved in bone resorption . Additionally, OPG’s interaction with TRAIL inhibits apoptosis in certain cells .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: OPG expression is regulated by various factors, including calcium influx through L-type voltage-sensitive calcium channels, 1,25-dihydroxyvitamin D3, and cytokines such as TNF-α and IL-1β . Transcriptional regulation involves the cAMP response element-binding protein, while post-translational modifications include glycosylation and disulfide bond formation .

Applications

Biomedical Research: OPG is extensively studied for its role in bone metabolism and its potential therapeutic applications in treating osteoporosis and other bone-related disorders .

Diagnostic Tools: OPG levels are measured as biomarkers for bone diseases, cardiovascular diseases, and certain cancers .

Therapeutic Strategies: OPG or its analogs are explored as potential treatments for osteoporosis, rheumatoid arthritis, and cancer due to their ability to inhibit bone resorption and tumor-induced bone destruction .

Role in the Life Cycle

Role Throughout the Life Cycle: OPG plays a vital role in bone development and maintenance throughout life. During development, it regulates bone density and prevents excessive bone resorption . In aging, OPG levels influence the risk of osteoporosis and other bone-related diseases . Additionally, OPG’s role in immune regulation and apoptosis inhibition impacts various physiological and pathological processes, including cancer and cardiovascular diseases .

© Copyright 2024 Thebiotek. All Rights Reserved.