Cytokines
Product List
TNF b Human, His
Tumor Necrosis Factor-Beta Human Recombinant, His Tag
TNF B Human, Sf9
Tumor Necrosis Factor-beta Human Recombinant, Sf9
TNFA Bovine
Tumor Necrosis Factor-alpha Bovine Recombinant
Escherichia Coli.
TNFR2 Human
Tumor Necrosis Factor Receptor Type 2 Human Recombinant
TNFR2 Human Fc
Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant
TNFR2 Human, His
Tumor Necrosis Factor Receptor Type 2 Human Recombinant, His Tag
TNFR2 Human, Sf9
Tumor Necrosis Factor Receptor Type 2 Human Recombinant, Sf9
TNFRSF17 Human, Sf9
B-Cell Maturation Antigen, Sf9 Human Recombinant
Recombinant human TNFRSF17 protein is produced in Baculovirus (Sf9 insect cells) as a single glycosylated polypeptide chain. It consists of 296 amino acids, including amino acids 1-54, and has a molecular mass of 33.1 kDa. TNFRSF17 is fused to a 242 amino acid hIgG-His-Tag at the C-terminus and is purified using proprietary chromatographic techniques.
Sf9, Baculovirus cells.
TNFRSF21 Human
TNF Ligand Receptor Superfamily Member 21 Human Recombinant
Produced in Sf9 insect cells using a baculovirus expression system, TNFRSF21 is a single, glycosylated polypeptide chain consisting of 547 amino acids (residues 42-349a.a.). It has a molecular weight of 60.4 kDa, although it may appear between 50-70 kDa on SDS-PAGE due to glycosylation. The protein is expressed with a C-terminal 239 amino acid His-tag to facilitate purification by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
The product is a sterile-filtered, colorless solution.
TNFRSF25 Human
TNF Ligand Receptor Superfamily Member 25 Recombinant Human
Introduction
Tumor Necrosis Factor (TNF) is a cytokine, a type of signaling protein involved in systemic inflammation and is part of the body’s immune response. TNF is primarily produced by activated macrophages, although it can also be produced by other cell types such as lymphocytes, natural killer cells, and neurons. TNF is classified into two main forms:
- TNF-α (Tumor Necrosis Factor-alpha): The most studied form, involved in systemic inflammation and acute phase reactions.
- TNF-β (Tumor Necrosis Factor-beta): Also known as lymphotoxin, it is produced by lymphocytes and has similar but distinct functions compared to TNF-α.
Key Biological Properties:
- Molecular Weight: TNF-α is a 17 kDa protein, while TNF-β is slightly larger.
- Structure: TNF-α is a trimeric protein, meaning it forms a complex of three identical subunits.
Expression Patterns:
- TNF-α: Expressed primarily by macrophages, but also by other immune cells such as T cells and natural killer cells.
- TNF-β: Expressed by activated lymphocytes.
Tissue Distribution:
- TNF is found in various tissues, including the spleen, liver, and adipose tissue. It is also present in the bloodstream during systemic inflammation.
Primary Biological Functions:
- Inflammation: TNF is a key mediator of inflammation, promoting the recruitment of immune cells to sites of infection or injury.
- Cell Death: TNF can induce apoptosis (programmed cell death) in certain cells, which is crucial for controlling infections and preventing cancer.
- Immune Response: TNF plays a role in the activation and differentiation of immune cells, enhancing the body’s ability to fight off pathogens.
Role in Immune Responses:
- Pathogen Recognition: TNF helps in recognizing and responding to pathogens by activating immune cells and promoting the production of other cytokines.
Mechanisms with Other Molecules and Cells:
- Receptors: TNF exerts its effects by binding to two receptors, TNFR1 and TNFR2, which are present on the surface of various cells.
- Binding Partners: TNF can interact with other cytokines and signaling molecules to amplify or modulate its effects.
Downstream Signaling Cascades:
- NF-κB Pathway: Activation of TNFR1 leads to the activation of the NF-κB pathway, which promotes the expression of genes involved in inflammation and cell survival.
- MAPK Pathway: TNF can also activate the MAPK pathway, leading to the production of inflammatory mediators.
Regulatory Mechanisms:
- Transcriptional Regulation: The expression of TNF is tightly regulated at the transcriptional level by various transcription factors, including NF-κB and AP-1.
- Post-Translational Modifications: TNF undergoes several post-translational modifications, such as glycosylation and cleavage, which can affect its activity and stability.
Biomedical Research:
- Disease Models: TNF is used in research to study inflammatory diseases, cancer, and autoimmune disorders.
Diagnostic Tools:
- Biomarkers: Elevated levels of TNF in the blood can serve as biomarkers for various inflammatory and autoimmune diseases.
Therapeutic Strategies:
- Anti-TNF Therapies: Drugs that inhibit TNF, such as infliximab and etanercept, are used to treat conditions like rheumatoid arthritis, Crohn’s disease, and psoriasis.
Role Throughout the Life Cycle:
- Development: TNF is involved in embryonic development, particularly in the formation of the immune system.
- Aging: TNF levels can increase with age, contributing to age-related inflammation and diseases.
- Disease: Dysregulation of TNF is associated with various diseases, including chronic inflammatory conditions, cancer, and neurodegenerative disorders.
