Cytokines
Product List
Recombinant Human Tumor necrosis factor ligand superfamily member 13B (TNFSF13B), partial (Active)
Our Recombinant Human TNFSF13B (CD257) protein is a valuable tool for cancer research and immunology investigations. TNFSF13B, also known as Tumor necrosis factor ligand superfamily member 13B or B-cell-activating factor (BAFF), is a critical cytokine involved in B-cell activation and survival. It plays a significant role in regulating B-cell development, proliferation, and antibody production, making it a key target for understanding immune responses and exploring therapeutic strategies.
Produced using our advanced E. coli expression system, this Tag-Free recombinant protein exhibits exceptional purity (>95% as determined by SDS-PAGE and HPLC) and low endotoxin levels (<1.0 EU/µg). The protein encompasses amino acids 134 to 285, representing a partial length of the TNFSF13B sequence. Its native structure is preserved, ensuring reliable and consistent results in your experiments.
Our TNFSF13B protein exhibits potent biological activity, demonstrating significant effects on B-cell survival and activation. Its activity has been validated through a mouse splenocyte survival assay, demonstrating an effective dose (ED50) ranging from 0.5 to 2 µg/ml. This lyophilized powder-form protein offers convenient use in diverse applications, benefiting from its long-term stability and ease of reconstitution. Uncover new insights into cancer biology and immunological processes with our high-quality TNFSF13B protein.
Recombinant Human CD40 ligand (CD40LG), partial (Active)
Recombinant Human CD40 ligand (CD40LG) is a valuable tool for cancer research, particularly in the field of immunology. CD40LG, also known as CD40 ligand or T-cell antigen Gp39, is a member of the tumor necrosis factor (TNF) ligand superfamily. It plays a crucial role in immune response regulation and activation of various immune cells.
This high-quality recombinant protein is derived from an E. coli expression system and encompasses amino acids 113 to 261 (C194W). Presented in a tag-free format, it ensures purity and minimizes potential interference from extraneous tags. With a purity exceeding 95%, as determined by SDS-PAGE and HPLC, this protein offers consistency and reliability for your experiments.
Our CD40LG exhibits full biological activity, comparable to a standard reference. It possesses an effective dose (ED50) of less than 5-10 ng/ml in the dose-dependent stimulation of interleukin-8 (IL-8) production by human peripheral blood mononuclear cells (PBMC). The product is supplied as a lyophilized powder, providing convenience and stability for long-term storage and ease of use in research applications.
Recombinant Human Tumor necrosis factor receptor superfamily member 17 protein (TNFRSF17), partial (Active)
This Recombinant Human TNFRSF17 protein is intended for cancer research. TNFRSF17, also known as Tumor necrosis factor receptor superfamily member 17 or B-cell maturation protein (BCMA), plays a crucial role in B-cell development and is implicated in various cancer-related processes.
Our protein is produced using an E. coli expression system and encompasses amino acids 5 to 54, representing a portion of the TNFRSF17 sequence. It is tag-free, ensuring its native conformation and eliminating potential interference in downstream applications. With a purity exceeding 98% and minimal endotoxin contamination, our TNFRSF17 protein guarantees reliable and consistent results.
The protein's activity has been validated. The lyophilized powder form ensures easy handling and storage, providing convenience for your research endeavors. Investigate the potential of TNFRSF17 in cancer biology and unravel its role in B-cell maturation and tumorigenesis with our high-quality and bioactive protein.
Recombinant Human Tumor necrosis factor protein (TNF), partial (Active)
Recombinant human tumor necrosis factor protein (TNF) is a partial-length protein comprising amino acids 77-233 of human TNF. Expressed in E. coli cells, this protein exhibits a purity exceeding 98%, as confirmed by SDS-PAGE and HPLC analysis. Functional characterization through a cytotoxicity assay using murine L929 cells demonstrates its full biological activity (ED50 <0.05 ng/ml, specific activity >2.0x107 IU/mg). Endotoxin levels are below 1.0 EU/µg, as determined by the LAL method. Our inventory includes ready-to-use TNF proteins, and custom tag modifications are available upon request. This recombinant TNF protein serves as a valuable tool for various applications, including specific antibody synthesis and cancer research.
TNF, also known as TNF-α, is a pro-inflammatory cytokine that plays a crucial role in the inflammatory response. Its pro-inflammatory effects are mediated via TNF-α/TNFR signaling. TNF is primarily produced by macrophages and is triggered in response to injury, bacterial or viral infections. Its function is to protect against infections and promote wound healing. However, excessive TNF expression can lead to excessive inflammation and may contribute to the development of autoimmune diseases. Several inflammatory autoimmune conditions, including psoriatic arthritis, rheumatoid arthritis (RA), and Crohn's disease, are linked to TNF. Anti-TNF therapy has become a standard clinical treatment for managing autoimmune diseases like RA. Furthermore, research has demonstrated that TNF can induce cell death in certain tumor cells.
Recombinant Human Tumor necrosis factor receptor superfamily member 11A protein (TNFRSF11A), partial (Active)
The Recombinant Human Tumor necrosis factor receptor superfamily member 11A protein (TNFRSF11A) is a partial-length protein encompassing amino acids 29-202 of the human TNFRSF11A. This protein was produced through the expression of a DNA fragment encoding this region in E. coli. Rigorous validation via SDS-PAGE and HPLC analyses confirmed a purity exceeding 90%. Furthermore, its biological activity was verified through its interaction with sRANK Ligand. The endotoxin level of this TNFRSF11A is below 1.0 EU/µg as determined by the LAL method. This recombinant TNFRSF11A protein is readily available, eliminating any waiting time for preparation. Custom tagged proteins are also offered upon request. This recombinant TNFRSF11A protein can be utilized for the development of specific antibodies or in research focusing on TNFRSF11A-associated cancers.
TNFRSF11A, also known as RANK, serves as the receptor for RANKL. The interaction between RANK and RANKL plays a critical role in a multitude of biological processes, including bone resorption and remodeling, bone marrow formation, development of the thymus and lymph nodes, intestinal immunity, inflammatory responses within the skin and central nervous system (CNS), tumorigenesis, and metastasis. Mutations within RANK or RANKL have been linked to the pathogenesis of bone diseases and certain types of cancer.
Recombinant Human Tumor necrosis factor receptor superfamily member 13C protein (TNFRSF13C), partial (Active)
This Recombinant Human TNFRSF13C (CD268) protein is a valuable tool for cancer research. TNFRSF13C, also known as Tumor necrosis factor receptor superfamily member 13C, plays a critical role in immune regulation and is implicated in various cancer-related processes.
Our TNFRSF13C protein is produced using an E. coli expression system and encompasses amino acids 1 to 76, representing a partial length of the TNFRSF13C sequence. It is tag-free, ensuring its native conformation and eliminating any potential interference in downstream applications. With a purity exceeding 95% and minimal endotoxin contamination, our TNFRSF13C protein provides reliable and consistent results.
Recombinant Human Tumor necrosis factor receptor superfamily member 13B protein (TNFRSF13B), partial (Active)
This Recombinant Human TNFRSF13B (CD267) protein is a valuable research tool in the field of cancer research. TNFRSF13B, also known as Transmembrane activator and CAML interactor (TACI), is a member of the tumor necrosis factor receptor superfamily and plays a crucial role in immune regulation and B-cell development.
Our protein, produced using an E. coli expression system, encompasses amino acids 1 to 160, representing a partial length of the TNFRSF13B sequence. It is tag-free, ensuring its native conformation and eliminating potential interference in downstream applications. With a purity exceeding 95% and minimal endotoxin contamination, our TNFRSF13B protein guarantees reliable and consistent results.
Demonstrating full biological activity when compared to standards, this protein effectively blocks human BAFF-induced T2B cell survival. Our protein exhibits potency in cancer-related studies, with a concentration range of 1.0-3.0 µg/ml. The lyophilized powder form simplifies handling and storage, offering convenience for your research endeavors.
Recombinant Human Tumor necrosis factor receptor superfamily member 1A protein (TNFRSF1A), partial (Active)
THE BioTek's Recombinant Human TNFRSF1A protein is a valuable reagent for cancer research. TNFRSF1A, also known as Tumor necrosis factor receptor superfamily member 1A or TNF-R1, is a crucial mediator of immune responses and inflammation, making it a significant target in cancer biology.
Produced using an E. coli expression system, our protein encompasses amino acids 22 to 211, representing a partial length of the TNFRSF1A sequence. It is tag-free, ensuring its native conformation and minimizing potential interference in downstream applications. With a purity exceeding 97% and minimal endotoxin contamination, our TNFRSF1A protein provides reliable and consistent results.
Validated for activity, our protein demonstrates its functional relevance in cancer research. The lyophilized powder form offers ease of handling and storage, enhancing convenience in your laboratory workflows. Delve into the intricate mechanisms of TNFRSF1A in cancer development and immune regulation with our high-quality and bioactive protein.
Introduction
Tumor Necrosis Factor (TNF) is a cytokine, a type of signaling protein involved in systemic inflammation and is part of the body’s immune response. TNF is primarily produced by activated macrophages, although it can also be produced by other cell types such as lymphocytes, natural killer cells, and neurons. TNF is classified into two main forms:
- TNF-α (Tumor Necrosis Factor-alpha): The most studied form, involved in systemic inflammation and acute phase reactions.
- TNF-β (Tumor Necrosis Factor-beta): Also known as lymphotoxin, it is produced by lymphocytes and has similar but distinct functions compared to TNF-α.
Key Biological Properties:
- Molecular Weight: TNF-α is a 17 kDa protein, while TNF-β is slightly larger.
- Structure: TNF-α is a trimeric protein, meaning it forms a complex of three identical subunits.
Expression Patterns:
- TNF-α: Expressed primarily by macrophages, but also by other immune cells such as T cells and natural killer cells.
- TNF-β: Expressed by activated lymphocytes.
Tissue Distribution:
- TNF is found in various tissues, including the spleen, liver, and adipose tissue. It is also present in the bloodstream during systemic inflammation.
Primary Biological Functions:
- Inflammation: TNF is a key mediator of inflammation, promoting the recruitment of immune cells to sites of infection or injury.
- Cell Death: TNF can induce apoptosis (programmed cell death) in certain cells, which is crucial for controlling infections and preventing cancer.
- Immune Response: TNF plays a role in the activation and differentiation of immune cells, enhancing the body’s ability to fight off pathogens.
Role in Immune Responses:
- Pathogen Recognition: TNF helps in recognizing and responding to pathogens by activating immune cells and promoting the production of other cytokines.
Mechanisms with Other Molecules and Cells:
- Receptors: TNF exerts its effects by binding to two receptors, TNFR1 and TNFR2, which are present on the surface of various cells.
- Binding Partners: TNF can interact with other cytokines and signaling molecules to amplify or modulate its effects.
Downstream Signaling Cascades:
- NF-κB Pathway: Activation of TNFR1 leads to the activation of the NF-κB pathway, which promotes the expression of genes involved in inflammation and cell survival.
- MAPK Pathway: TNF can also activate the MAPK pathway, leading to the production of inflammatory mediators.
Regulatory Mechanisms:
- Transcriptional Regulation: The expression of TNF is tightly regulated at the transcriptional level by various transcription factors, including NF-κB and AP-1.
- Post-Translational Modifications: TNF undergoes several post-translational modifications, such as glycosylation and cleavage, which can affect its activity and stability.
Biomedical Research:
- Disease Models: TNF is used in research to study inflammatory diseases, cancer, and autoimmune disorders.
Diagnostic Tools:
- Biomarkers: Elevated levels of TNF in the blood can serve as biomarkers for various inflammatory and autoimmune diseases.
Therapeutic Strategies:
- Anti-TNF Therapies: Drugs that inhibit TNF, such as infliximab and etanercept, are used to treat conditions like rheumatoid arthritis, Crohn’s disease, and psoriasis.
Role Throughout the Life Cycle:
- Development: TNF is involved in embryonic development, particularly in the formation of the immune system.
- Aging: TNF levels can increase with age, contributing to age-related inflammation and diseases.
- Disease: Dysregulation of TNF is associated with various diseases, including chronic inflammatory conditions, cancer, and neurodegenerative disorders.
