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Product List
CES2E Mouse
Carboxylesterase 2E Mouse Recombinant
Produced in Sf9 Baculovirus cells, CES2E Mouse is a single, glycosylated polypeptide chain with a molecular mass of 60.5kDa. It consists of 541 amino acids (27-559 aa) and is fused to an 8 amino acid His tag at the C-terminus. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs 
Cat. No.
BT5203
Source
Sf9,
Baculovirus cells.
Appearance
Sterile Filtered colorless solution.
Lipase A
Lipase-A Recombinant
Recombinant Immobilized Serratia marcescens Lipase-A, with a molecular weight of 65 kDa, is produced in E. coli bacteria and purified using standard techniques.
Shipped with Ice Packs
Cat. No.
BT5281
Source
Escherichia Coli.
Appearance
A sterile, filtered powder that has been freeze-dried.
LIPG Human
Lipase Endothelial Human Recombinant
Recombinant Human LIPG, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 343 amino acids (residues 21-340) with a molecular weight of 38kDa. This protein features a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5349
Source
Escherichia Coli.
Appearance
Clear, sterile solution after filtration.
Introduction
Definition and Classification
Lipases are a group of enzymes that catalyze the hydrolysis of fats (lipids) into glycerol and free fatty acids. They are classified based on their source and substrate specificity:
- Pancreatic Lipase: Found in the pancreas, it plays a crucial role in the digestion of dietary fats.
- Hepatic Lipase: Located in the liver, it is involved in the metabolism of lipoproteins.
- Hormone-Sensitive Lipase: Present in adipose tissue, it is regulated by hormonal signals and is essential for mobilizing stored fats.
- Lipoprotein Lipase: Found in the endothelial cells of capillaries, it hydrolyzes triglycerides in lipoproteins.
Biological Properties
Key Biological Properties:
- Catalytic Activity: Lipases exhibit high specificity for the ester bonds in triglycerides.
- Stability: They are stable under a wide range of pH and temperature conditions.
Expression Patterns:
- Pancreatic Lipase: Expressed predominantly in the pancreas.
- Hepatic Lipase: Expressed in the liver.
- Hormone-Sensitive Lipase: Expressed in adipose tissue.
- Lipoprotein Lipase: Expressed in endothelial cells of capillaries.
Tissue Distribution:
- Pancreatic Lipase: Found in the pancreas and small intestine.
- Hepatic Lipase: Found in the liver and blood plasma.
- Hormone-Sensitive Lipase: Found in adipose tissue.
- Lipoprotein Lipase: Found in capillary endothelial cells.
Biological Functions
Primary Biological Functions:
- Fat Digestion: Pancreatic lipase breaks down dietary fats into absorbable units.
- Lipoprotein Metabolism: Hepatic and lipoprotein lipases are involved in the metabolism of lipoproteins, which transport fats in the blood.
- Fat Mobilization: Hormone-sensitive lipase mobilizes stored fats for energy production.
Role in Immune Responses:
- Pathogen Recognition: Lipases can recognize and hydrolyze lipid components of pathogen membranes, aiding in immune defense.
Modes of Action
Mechanisms with Other Molecules and Cells:
- Binding to Substrates: Lipases bind to triglycerides and hydrolyze the ester bonds.
- Interaction with Co-factors: Some lipases require co-factors such as bile salts for optimal activity.
Binding Partners:
- Colipase: Pancreatic lipase requires colipase for binding to lipid-water interfaces.
- Apolipoproteins: Lipoprotein lipase interacts with apolipoproteins on lipoprotein particles.
Downstream Signaling Cascades:
- Hormone-Sensitive Lipase: Activated by cyclic AMP (cAMP) signaling in response to hormonal signals like adrenaline.
Regulatory Mechanisms
Regulatory Mechanisms:
- Transcriptional Regulation: Gene expression of lipases is regulated by transcription factors such as PPARs (Peroxisome Proliferator-Activated Receptors).
- Post-Translational Modifications: Lipases can be activated or inhibited by phosphorylation, glycosylation, and other modifications.
Applications
Biomedical Research:
- Metabolic Studies: Lipases are used to study lipid metabolism and related disorders.
Diagnostic Tools:
- Enzyme Assays: Lipase activity assays are used to diagnose pancreatic diseases.
Therapeutic Strategies:
- Enzyme Replacement Therapy: Lipase supplements are used to treat conditions like exocrine pancreatic insufficiency.
Role in the Life Cycle
Role Throughout the Life Cycle:
- Development: Lipases are essential for the digestion and absorption of dietary fats, crucial for growth and development.
- Aging: Changes in lipase activity can affect lipid metabolism and contribute to age-related diseases.
- Disease: Dysregulation of lipase activity is associated with metabolic disorders such as obesity, diabetes, and cardiovascular diseases.
