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Product List
POLR2J2 Human
Polymerase II Polypeptide J2 Human Recombinant
Recombinant human POLR2J2, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 15.5 kDa. It consists of 138 amino acids (residues 1-115) and includes a 23 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
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PARP1 Human
Poly (ADP-Ribose) Polymerase 1 Human Recombinant
Recombinant human PARP1, expressed in E. coli, is a non-glycosylated polypeptide chain comprising amino acids 662-1014 of the full PARP1 protein sequence. This truncated form of PARP1 retains its catalytic activity and is commonly used for in vitro studies. The protein has a molecular weight of approximately 39.6 kDa and is purified to high purity using proprietary chromatographic techniques.
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Cat. No.
BT2769
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.
PARP2 Human
Poly (ADP-Ribose) Polymerase 2 Human Recombinant
Recombinant human PARP2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 376 amino acids (233-583a.a), resulting in a molecular weight of 42.5 kDa. A 25 amino acid His-tag is fused to the N-terminus of PARP2. The protein undergoes purification using proprietary chromatographic methods.
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Cat. No.
BT2830
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
POLL Human
Polymerase (DNA directed), Lambda Human Recombinant
Recombinant human POLL, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 320 amino acids (residues 1-300) with a molecular weight of 36.0 kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Introduction
Definition and Classification
Polymerases are enzymes that synthesize long chains of polymers or nucleic acids. They play a crucial role in the replication and transcription of genetic material. There are two main types of polymerases:
Biological Properties
Key Biological Properties:
- DNA Polymerase: Essential for DNA replication, repair, and recombination. It adds nucleotides to the 3’ end of a DNA strand, ensuring accurate copying of genetic information .
- RNA Polymerase: Catalyzes the synthesis of RNA from a DNA template during transcription .
Expression Patterns and Tissue Distribution:
- DNA polymerases are ubiquitously expressed in all dividing cells, with higher expression in tissues with rapid cell turnover, such as the bone marrow and gastrointestinal tract .
- RNA polymerases are also widely expressed, with specific types (Pol I, Pol II, Pol III) having distinct roles in different tissues .
Biological Functions
Modes of Action
Mechanisms with Other Molecules and Cells:
- DNA polymerases interact with various proteins involved in the replication fork, such as helicases and primases .
- RNA polymerases interact with transcription factors and other regulatory proteins to initiate and regulate transcription .
Binding Partners and Downstream Signaling Cascades:
Regulatory Mechanisms
Applications
Biomedical Research:
- Polymerases are essential tools in molecular biology techniques such as PCR (Polymerase Chain Reaction) and DNA sequencing .
Diagnostic Tools:
- PCR-based diagnostic tests for infectious diseases and genetic disorders rely on the activity of DNA polymerases .
Therapeutic Strategies:
Role in the Life Cycle
Throughout Development to Aging and Disease:
- DNA polymerases are crucial for cell division and development, ensuring accurate DNA replication .
- RNA polymerases are involved in the transcription of genes necessary for growth, development, and response to environmental changes .
- Dysregulation of polymerase activity can lead to diseases such as cancer and genetic disorders .
