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Product List
DUSP6 Human
Dual Specificity Phosphatase 6 Human Recombinant
Recombinant human DUSP6 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 401 amino acids (with amino acids 1-381 present) and has a molecular weight of 44.4 kDa. A 20 amino acid His-tag is fused to the N-terminus of DUSP6. Purification is done using proprietary chromatographic techniques.
Shipped with Ice Packs 
Cat. No.
BT28317
Source
Escherichia Coli.
Appearance
A clear and colorless solution, sterilized by filtration.
DUT Human
Deoxyuridine Triphosphatase Human Recombinant
This product is a recombinant human DUT protein. It is produced in E. coli and includes a 21 amino acid His tag at the N-terminus. This single, non-glycosylated polypeptide chain contains 204 amino acids (70-252 a.a.) and has a molecular weight of 21.6 kDa. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT28379
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Shipped with Ice Packs
Cat. No.
BT28467
Source
Escherichia Coli.
Appearance
The enzyme is provided in a liquid form that has undergone sterile filtration.
ENOPH1 Human
Enolase-Phosphatase-1 Human Recombinant
Recombinant human ENOPH1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein construct consists of 281 amino acids, including a 20 amino acid His tag at the N-terminus (1-261 a.a. of the ENOPH1 sequence), resulting in a molecular mass of 31 kDa. Purification of ENOPH1 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28574
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.
ACP5 Human
Acid Phosphatase-5 Human Recombinant
Recombinant human ACP5, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 304 amino acids (22-325 a.a.) and has a molecular weight of 34.3kDa. Under reducing conditions on SDS-PAGE, it migrates between 28-40kDa.
The purification of ACP5 is achieved using proprietary chromatographic techniques.
The purification of ACP5 is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26476
Source
Sf9,
Baculovirus cells.
Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
ACP5 Human, His
Acid Phosphatase-5 Human Recombinant, His Tag
Recombinant human ACP5, expressed in Sf9 insect cells using a baculovirus system, is a single glycosylated polypeptide chain. It consists of 310 amino acids (residues 22-325), resulting in a molecular weight of 35.1 kDa. The protein includes a 6-amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26558
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless solution, sterile-filtered.
ACP6 Human
Acid Phosphatase-6 Human Recombinant
Recombinant human ACP6, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein comprises 419 amino acids (residues 33-428a.a), resulting in a molecular weight of 47.7 kDa. It includes a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26647
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.
DUSP10 Human
Dual Specificity Phosphatase 10 Human Recombinant
Recombinant human DUSP10, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This 359-amino acid protein (residues 149-482) has a molecular weight of 40.4 kDa. For purification, DUSP10 is tagged with a 25-amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27378
Source
E.coli.
Appearance
Clear, colorless, and sterile-filtered solution.
DUSP21 Human
Dual Specificity Phosphatase 21 Human Recombinant
Recombinant human DUSP21, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 214 amino acids (residues 1-190) and possessing a molecular weight of 24.1 kDa. This protein includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT27858
Source
E.coli.
Appearance
A sterile, colorless solution that has been filtered.
PDXP Human
Pyridoxal Phosphatase Human Recombinant
Recombinant Human PDXP, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 316 amino acids (residues 1-296) and possessing a molecular weight of 33.8 kDa. A 20 amino acid His-Tag is fused to the N-terminus of the PDXP protein. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT29669
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Introduction
Definition and Classification
Phosphatases are a group of enzymes that catalyze the removal of phosphate groups from molecules, a process known as dephosphorylation. They play a crucial role in various cellular processes by regulating the phosphorylation state of proteins and other molecules. Phosphatases are broadly classified into two main categories:
- Protein Phosphatases: These enzymes specifically target phosphorylated amino acid residues in proteins. They are further divided into:
- Serine/Threonine Phosphatases: Target serine or threonine residues.
- Tyrosine Phosphatases: Target tyrosine residues.
- Dual-Specificity Phosphatases: Can target both serine/threonine and tyrosine residues.
- Non-Protein Phosphatases: These enzymes act on non-protein substrates, such as nucleotides, sugars, and lipids.
Biological Properties
Key Biological Properties:
- Catalytic Activity: Phosphatases hydrolyze phosphate esters, releasing inorganic phosphate.
- Substrate Specificity: They exhibit specificity for their substrates, which can be proteins, nucleotides, or other molecules.
Expression Patterns:
- Phosphatases are ubiquitously expressed in various tissues and cell types, with specific isoforms showing distinct expression patterns.
Tissue Distribution:
- Protein Phosphatases: Widely distributed across tissues, with high expression in the brain, liver, and muscles.
- Non-Protein Phosphatases: Found in various tissues, depending on their specific substrates.
Biological Functions
Primary Biological Functions:
- Regulation of Signal Transduction: Phosphatases modulate signaling pathways by dephosphorylating key signaling molecules.
- Cell Cycle Control: They play a role in cell cycle progression by regulating the phosphorylation state of cell cycle proteins.
- Metabolic Regulation: Phosphatases are involved in metabolic pathways by dephosphorylating metabolic enzymes.
Role in Immune Responses:
- Phosphatases regulate immune cell activation and function by modulating signaling pathways involved in immune responses.
Pathogen Recognition:
- Some phosphatases are involved in recognizing and responding to pathogen-associated molecular patterns (PAMPs), contributing to the immune defense.
Modes of Action
Mechanisms with Other Molecules and Cells:
- Phosphatases interact with various molecules, including proteins, lipids, and nucleotides, to exert their dephosphorylation activity.
Binding Partners:
- They often form complexes with other proteins, which can regulate their activity and substrate specificity.
Downstream Signaling Cascades:
- By dephosphorylating key signaling molecules, phosphatases influence downstream signaling pathways, affecting cellular responses such as proliferation, differentiation, and apoptosis.
Regulatory Mechanisms
Regulatory Mechanisms:
- Transcriptional Regulation: The expression of phosphatases is regulated at the transcriptional level by various transcription factors and signaling pathways.
- Post-Translational Modifications: Phosphatases themselves can be regulated by post-translational modifications, such as phosphorylation, ubiquitination, and methylation, which can alter their activity, stability, and localization.
Applications
Biomedical Research:
- Phosphatases are studied to understand their role in various diseases, including cancer, diabetes, and neurodegenerative disorders.
Diagnostic Tools:
- Phosphatase activity assays are used in diagnostic tests to measure enzyme activity in biological samples, aiding in the diagnosis of certain diseases.
Therapeutic Strategies:
- Inhibitors of specific phosphatases are being developed as potential therapeutic agents for diseases where phosphatase activity is dysregulated.
Role in the Life Cycle
Role Throughout the Life Cycle:
- Development: Phosphatases are involved in embryonic development by regulating signaling pathways that control cell differentiation and tissue formation.
- Aging: Changes in phosphatase activity have been associated with aging and age-related diseases, such as Alzheimer’s disease.
- Disease: Dysregulation of phosphatase activity is implicated in various diseases, including cancer, where altered phosphorylation states can lead to uncontrolled cell growth and proliferation.
