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Product List
ENPP1 Human
Ectonucleotide Pyrophosphatase Human Recombinant
This section describes the specific features of the recombinant ENPP1 protein product, including its source, structure, molecular weight, and purification method.
Shipped with Ice Packs 
Cat. No.
BT28634
Source
HEK 293.
Appearance
Describes the product's appearance after the freeze-drying process.
ENPP1 Mouse
Ectonucleotide Pyrophosphatase Mouse Recombinant
ENPP1 Mouse Recombinant, produced in HEK cells, is a single, glycosylated polypeptide chain containing 828 amino acids (Lys85-Glu906) with a molecular mass of 95.2 kDa. This protein is fused to a 6 a.a his tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28716
Source
HEK 293.
Appearance
Filtered white lyophilized (freeze-dried) powder.
ENPP2 Human
Ectonucleotide Pyrophosphatase-2 Human Recombinant
Recombinant human ENPP2, expressed in HEK293 cells, is a single, glycosylated polypeptide chain with a molecular weight of 94.9 kDa. The protein consists of 825 amino acids (residues 49-863) and includes a C-terminal 6-histidine tag for purification purposes. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
PHOSPHO1 Human
Phosphatase Orphan-1 Human Recombinant
Recombinant Human Phospho1, produced in E.Coli, is a single, non-glycosylated polypeptide chain comprising 295 amino acids. With a molecular weight of 31.3 kDa, it features a 14 aa His tag at the N-Terminus. Purification of Human Phosphocholine Phosphatase is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29849
Source
Escherichia Coli.
Appearance
It appears as a filtered, lyophilized (freeze-dried) powder.
PHOSPHO2 Human
Phosphatase Orphan-2 Human Recombinant
Recombinant human PHOSPHO2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 265 amino acids, with a molecular weight of 30.3 kDa. The protein includes the core PHOSPHO2 sequence (amino acids 1-241) and an N-terminal 24 amino acid His-tag. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT29921
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.
PHPT1 Human
Phosphohistidine Phosphatase 1 Human Recombinant
Produced in E. coli, PHPT1 is a single, non-glycosylated polypeptide chain consisting of 145 amino acids (1-125a.a.) with a molecular weight of 15.9 kDa. It includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
ACPP Human
Acid Phosphatase Prostate Human Recombinant
Recombinant human ACPP, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 375 amino acids (residues 33-386). It has a molecular weight of 43.2 kDa. The recombinant protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT26814
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.
ACPP Human, Sf9
Acid Phosphatase Prostate, Human Recombinant, sf9
ACPP, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 41.8kDa (360 amino acids, 33-386 a.a.). It appears as a band between 40-57kDa on SDS-PAGE under reducing conditions. This ACPP protein is expressed with a 6 amino acid His tag at its C-terminus and purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT26918
Source
Sf9,
Baculovirus cells.
Appearance
A clear, sterile-filtered solution with no color.
ACPP Mouse
Acid Phosphatase Prostate Mouse Recombinant
ACPP Mouse, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 356 amino acids (32-381 aa) and has a molecular weight of 41.3kDa. The protein includes a 6 amino acid His tag fused at the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27003
Source
Sf9,
Baculovirus cells.
Appearance
A clear, colorless solution, sterilized by filtration.
DUSP3 Human
Dual Specificity Phosphatase 3 Human Recombinant
Recombinant human DUSP3, produced in E.coli, is a non-glycosylated polypeptide chain consisting of 205 amino acids (residues 1-185). It has a molecular weight of 22.6 kDa. The protein is expressed with an N-terminal 20 amino acid His-tag and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28235
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Introduction
Definition and Classification
Phosphatases are a group of enzymes that catalyze the removal of phosphate groups from molecules, a process known as dephosphorylation. They play a crucial role in various cellular processes by regulating the phosphorylation state of proteins and other molecules. Phosphatases are broadly classified into two main categories:
- Protein Phosphatases: These enzymes specifically target phosphorylated amino acid residues in proteins. They are further divided into:
- Serine/Threonine Phosphatases: Target serine or threonine residues.
- Tyrosine Phosphatases: Target tyrosine residues.
- Dual-Specificity Phosphatases: Can target both serine/threonine and tyrosine residues.
- Non-Protein Phosphatases: These enzymes act on non-protein substrates, such as nucleotides, sugars, and lipids.
Biological Properties
Key Biological Properties:
- Catalytic Activity: Phosphatases hydrolyze phosphate esters, releasing inorganic phosphate.
- Substrate Specificity: They exhibit specificity for their substrates, which can be proteins, nucleotides, or other molecules.
Expression Patterns:
- Phosphatases are ubiquitously expressed in various tissues and cell types, with specific isoforms showing distinct expression patterns.
Tissue Distribution:
- Protein Phosphatases: Widely distributed across tissues, with high expression in the brain, liver, and muscles.
- Non-Protein Phosphatases: Found in various tissues, depending on their specific substrates.
Biological Functions
Primary Biological Functions:
- Regulation of Signal Transduction: Phosphatases modulate signaling pathways by dephosphorylating key signaling molecules.
- Cell Cycle Control: They play a role in cell cycle progression by regulating the phosphorylation state of cell cycle proteins.
- Metabolic Regulation: Phosphatases are involved in metabolic pathways by dephosphorylating metabolic enzymes.
Role in Immune Responses:
- Phosphatases regulate immune cell activation and function by modulating signaling pathways involved in immune responses.
Pathogen Recognition:
- Some phosphatases are involved in recognizing and responding to pathogen-associated molecular patterns (PAMPs), contributing to the immune defense.
Modes of Action
Mechanisms with Other Molecules and Cells:
- Phosphatases interact with various molecules, including proteins, lipids, and nucleotides, to exert their dephosphorylation activity.
Binding Partners:
- They often form complexes with other proteins, which can regulate their activity and substrate specificity.
Downstream Signaling Cascades:
- By dephosphorylating key signaling molecules, phosphatases influence downstream signaling pathways, affecting cellular responses such as proliferation, differentiation, and apoptosis.
Regulatory Mechanisms
Regulatory Mechanisms:
- Transcriptional Regulation: The expression of phosphatases is regulated at the transcriptional level by various transcription factors and signaling pathways.
- Post-Translational Modifications: Phosphatases themselves can be regulated by post-translational modifications, such as phosphorylation, ubiquitination, and methylation, which can alter their activity, stability, and localization.
Applications
Biomedical Research:
- Phosphatases are studied to understand their role in various diseases, including cancer, diabetes, and neurodegenerative disorders.
Diagnostic Tools:
- Phosphatase activity assays are used in diagnostic tests to measure enzyme activity in biological samples, aiding in the diagnosis of certain diseases.
Therapeutic Strategies:
- Inhibitors of specific phosphatases are being developed as potential therapeutic agents for diseases where phosphatase activity is dysregulated.
Role in the Life Cycle
Role Throughout the Life Cycle:
- Development: Phosphatases are involved in embryonic development by regulating signaling pathways that control cell differentiation and tissue formation.
- Aging: Changes in phosphatase activity have been associated with aging and age-related diseases, such as Alzheimer’s disease.
- Disease: Dysregulation of phosphatase activity is implicated in various diseases, including cancer, where altered phosphorylation states can lead to uncontrolled cell growth and proliferation.
