Recombinant Proteins
Product List
ZNF32 Human
Zinc Finger Protein 32 Human Recombinant
ZNF514 Human
Zinc Finger Protein 514 Human Recombinant
ZCCHC12 Human
Zinc Finger, CCHC Domain Containing 12 Human Recombinant
ZCCHC17 Human
Zinc Finger, CCHC Domain Containing 17 Human Recombinant
ZFAND3 Human
Zinc Finger, AN1-Type Domain 3 Human Recombinant
ZFAND5 Human
Zinc Finger, AN1-Type Domain 5 Human Recombinant
This ZFAND5 protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Introduction
Zinc fingers are small protein structural motifs characterized by the coordination of one or more zinc ions (Zn²⁺) which stabilize the fold . These motifs were first identified in the transcription factor IIIA from the African clawed frog (Xenopus laevis) . Zinc fingers are classified into several structural families based on their three-dimensional architecture and the identity of the ligands coordinating the zinc ion. The most common types include C2H2, C4, and C6 zinc fingers .
Zinc finger proteins (ZFPs) are ubiquitous in eukaryotic cells and play crucial roles in various biological processes. They are primarily found in the nucleus, where they function as transcription factors . ZFPs exhibit diverse expression patterns and tissue distribution, being present in almost all tissues but with varying abundance . They are involved in gene regulation, cell differentiation, and embryonic development .
ZFPs primarily function as transcription factors, regulating the expression of genes by binding to specific DNA sequences . They play significant roles in immune responses and pathogen recognition by regulating the transcription of genes involved in these processes . Additionally, ZFPs are implicated in cell differentiation, embryonic development, and various diseases, including cancers .
Zinc fingers interact with DNA, RNA, proteins, and other small molecules . They bind to specific DNA sequences through their finger-like protrusions, facilitating the regulation of gene transcription . ZFPs can also interact with other proteins to form complexes that modulate downstream signaling cascades . These interactions are crucial for the regulation of various cellular processes, including metabolism, autophagy, and apoptosis .
The expression and activity of ZFPs are tightly regulated at both the transcriptional and post-transcriptional levels . Transcriptional regulation involves the binding of ZFPs to promoter regions of target genes, modulating their expression . Post-translational modifications, such as phosphorylation and ubiquitination, further regulate the activity and stability of ZFPs . These regulatory mechanisms ensure precise control over the biological functions of ZFPs.
Zinc fingers have become invaluable tools in biomedical research and therapeutic strategies. Engineered zinc finger nucleases (ZFNs) are used for gene editing, allowing precise modifications of the genome . ZFPs are also employed in diagnostic tools to detect specific DNA sequences associated with diseases . Additionally, ZFPs hold potential as therapeutic agents for treating genetic disorders and cancers .
ZFPs play essential roles throughout the life cycle, from development to aging and disease . During embryonic development, ZFPs regulate the expression of genes involved in cell differentiation and organogenesis . In adulthood, they continue to modulate gene expression in response to various stimuli, maintaining cellular homeostasis . Dysregulation of ZFPs is associated with aging and the development of diseases, including neurodegenerative disorders and cancers .
