Recombinant Human coronavirus NL63 Non-structural protein 3 (NSP3) is produced using established recombinant DNA technology. A DNA template encoding NSP3, incorporating an N-terminal 10xHis tag, is constructed. This template enables efficient production of the recombinant protein. CUSABIO employs rigorous quality control (QC) procedures to ensure high quality. The expressed region corresponds to amino acids 1-225 of the HCoV-NL63 NSP3. Human coronavirus NL63 NSP3 is a viral protease crucial for viral protein biogenesis, replication/transcription complex (RTC) formation, and regulation of host ubiquitination and ISGylation. It plays multiple roles in the viral life cycle, functioning as a scaffold protein through self-interaction and interactions with other viral NSPs and host proteins.