Recombinant Proteins
Product List
Recombinant Human Interleukin-7 (IL7)
Recombinant human interleukin-7 (IL7) is produced using recombinant DNA technology. This involves co-inserting the DNA fragment encoding the 26-177aa of IL7 protein with the N-terminal 6xHis-SUMO-tag gene into an expression vector. The recombinant vector is then cultured in vitro using an E.coli expression system for protein expression. The recombinant human IL7 is harvested from the cell culture supernatant and undergoes affinity chromatography purification. Its purity reaches up to 90%, as determined by SDS-PAGE.
Human IL7 is a crucial hematopoietic growth factor involved in various immune processes. It plays a vital role in the development of thymic T cells, lymphoid homeostasis, and peripheral T cell survival [1]. IL7 is expressed in human intestinal epithelial cells and contributes to the functional differentiation of CD4+ T cells, impacting mucosal immunity [2]. IL7 also influences the differentiation of human neural progenitor cells, suggesting its involvement in neuronal tissue development and plasticity [3]. The aberrant expression of IL7 isoforms is found in human cancer tissues, highlighting its potential functions as tumor growth and differentiation factors [4].
References:
[1] R. O’Connell, A. Balazs, D. Rao, C. Kivork, L. Yang, & D. Baltimore, Lentiviral vector delivery of human interleukin-7 (hil-7) to human immune system (his) mice expands t lymphocyte populations, Plos One, vol. 5, no. 8, p. e12009, 2010. https://doi.org/10.1371/journal.pone.0012009
[2] K. Katamura, T. Fukui, T. Kiyomasu, K. Ohmura, J. Iio, & H. Ueno, Regulation of cd31 expression and interleukin‐4 production by human cord blood cd4+ t cells with interleukin‐4 and interleukin‐7, Pediatrics International, vol. 42, no. 2, p. 126-133, 2000. https://doi.org/10.1046/j.1442-200x.2000.01194.x
[3] M. Moors, N. Vudattu, J. Abel, U. Krämer, L. Rane, N. Ulfiget al., Interleukin-7 (il-7) and il-7 splice variants affect differentiation of human neural progenitor cells, Genes and Immunity, vol. 11, no. 1, p. 11-20, 2009. https://doi.org/10.1038/gene.2009.77
[4] N. Vudattu, I. Magalhaes, H. Hoehn, D. Pan, & M. Maeurer, Expression analysis and functional activity of interleukin-7 splice variants, Genes and Immunity, vol. 10, no. 2, p. 132-140, 2008. https://doi.org/10.1038/gene.2008.90
