Recombinant Proteins
Product List
Recombinant Human ATP-sensitive inward rectifier potassium channel 10 (KCNJ10)
Recombinant Human KCNJ10 protein is a full-length protein expressed in vitro using a cell-free E. coli system. Cell-free expression utilizes cell extracts containing all necessary components for transcription, translation, and post-translational modification. Supplementing with cofactors allows for rapid KCNJ10 protein synthesis within hours. While convenient for smaller-scale experiments, this method is not ideal for large-scale production. Key advantages include protein synthesis without cell culturing and the ability to co-express multiple proteins.
KCNJ10 is predominantly expressed in glial cells of the brain, inner ear, and kidney. In glial cells, KCNJ10 channels are crucial for extracellular potassium buffering, glutamate uptake, astrocyte development, and myelination. Within the inner ear, it regulates potassium homeostasis, contributing to endocochlear potential generation and maintenance—essential for hearing and cochlear development. In the kidney's distal convoluted tubules (DCTs), KCNJ10 facilitates potassium recycling and the establishment of a negative membrane potential. Autosomal recessive KCNJ10 gene mutations cause SeSAME/EAST syndrome, a multisystemic disorder.
