Recombinant Proteins
Product List
Recombinant Mouse Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (Cmtr2)
The recombinant Mouse Cmtr2 protein is produced using a cell-free in vitro expression system derived from *E. coli*. This system allows for the synthesis of the full-length protein without the need for cell culture. The cell-free extract contains all the necessary components for transcription, translation, and post-translational modifications of the recombinant protein. By supplementing with cofactors, Cmtr2 proteins can be generated within a few hours. However, this system is not suitable for large-scale production of recombinant proteins. Its advantages include the ability to synthesize proteins without cell culturing and the potential to express multiple proteins simultaneously.
Cmtr2 is a second transcribed nucleotide ribose O-2 methyltransferase found in both the nucleus and cytoplasm of MCF7 cells. It exhibits methyltransferase activity on the second transcribed nucleotide (cap2) at the 2'-prime-O-ribose position of capped mRNA and small nuclear RNA. Research has shown that Cmtr2 can methylate substrates, regardless of the methylation status of the cap guanosine or N1 ribose. Mutations in Cmtr2 have been identified in patients with lung adenocarcinomas.
