Recombinant Proteins
Product List
Recombinant Yersinia enterocolitica Outer membrane virulence protein YopE (yopE)
This product represents a recombinant Yersinia enterocolitica outer membrane virulence protein YopE (1-219aa) with an N-terminal 6xHis tag. The gene sequence encoding YopE is incorporated into a plasmid that includes a promoter, ribosome binding site (RBS), and terminator sequence for efficient expression. This plasmid is then linearized using restriction enzymes.
The recombinant YopE protein is produced using a cell-free expression system. This involves adding substrates, energy, amino acids, nucleotides, cofactors, salts, and an inducer IPTG to an E. coli cell-free extract. The inducer triggers the promoter, initiating transcription and translation of the yopE gene. The protein synthesis is monitored, and the reaction is harvested when the desired protein yield is achieved.
The harvested reaction mixture undergoes affinity chromatography to purify the YopE protein. The purified protein is then analyzed using SDS-PAGE to verify its identity and purity. The purity of the recombinant Yersinia enterocolitica YopE protein is over 90%.
