Recombinant Proteins
Product List
Recombinant Human 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4)
This recombinant human 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4) protein is produced in an in vitro E. coli expression system. The protein is tagged with an N-terminal 10xHis-tag. The full-length human AGPAT4 gene fragment (1-319aa) is cloned into a suitable expression vector, along with the N-terminal 10xHis-tag and necessary regulatory elements. The recombinant plasmid DNA containing the AGPAT4 gene fragment is then isolated and purified. In vitro transcription is performed using the purified plasmid DNA to synthesize mRNA, which acts as a template in the subsequent in vitro translation reaction. The translation reaction is carried out using an E. coli cell-free extract supplemented with energy sources and amino acids. After incubation, the recombinant AGPAT4 protein is purified from the cell culture medium. The purity of the recombinant AGPAT4 protein, reaching up to 85%, is assessed by SDS-PAGE analysis, where it appears as a band with a molecular weight of approximately 40 kDa.
