Recombinant Proteins
Product List
Recombinant Mouse Beta-tectorin (Tectb)
The cDNA fragment encoding amino acids 18-305 of Mouse Beta-tectorin/Tectb was fused with an N-terminal 6xHis-tag and subsequently expressed in an in vitro E.coli expression system. The resulting product is the recombinant full-length mature Mouse Tectb protein. The purity of this protein was determined using SDS-PAGE and reached up to 90%. On the reducing SDS-PAGE gel, a molecular mass band of approximately 38 kDa is observed. The slightly higher molecular weight is attributed to glycosylation. This recombinant Tectb protein can be utilized for the production of specific antibodies or for research involving the tectorial membrane (TM).
Tectb is a non-collagenous glycoprotein localized to the TM. Its expression is exclusively and highly concentrated in the inner ear. The absence of Tecb impairs the core structure of the TM and significantly alters cochlear function. Mammalian hearing is characterized by remarkable sensitivity and exquisite frequency selectivity. Roozbeh Ghaffari et al. demonstrated that mutations in Tectb lead to a reduction in the spatial extent and propagation velocity of TM traveling waves, which is likely responsible for the hearing abnormalities associated with these mutations. The deletion of the tectb gene in mouse models resulted in decreased sensitivity and sharper frequency selectivity.
